Molecular phylogeny of Rasaceae based on S-RNase related and organelle DNA polymorphism

基于S-RNase相关和细胞器DNA多态性的蔷薇科分子系统发育

• 批准号: 07660032
• 负责人: MATSUMOTO Shogo
• 金额: $ 1.47万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660032/
• 关键词: Rose; Apple; organelle; self-incompatibility; Molecular taxonomy; RFLP; PCR

(1) The results demonstrate the maternal inheritance of organelle DNA in rose and apple.(2) Phylogenetic analysis among 19 Malus species were carried out using the RFLP analysis of organelle DNA.The molecular classification conformed closely to the traditional classification, but some species from one series belonged to different clades on phylogenetic trees.(3) Phylogenetic analysis among 17 wild rose species were carried out using the RFLP analysis and matK sequence data of organelle DNA.Interspecific hybrids formed a clade with maternal parents. There was a phenotypical difference between some species forming a clade on phylogenetic trees and they belonged to different sections.(4) It was demonstrated that RAPD analysis is a useful technique for rose cultivars and clonal plants identification.(5) Malus species and cultivars were identified by RFLP analysis using S-RNase like genomic sequences in apple. The sequences are also useful for discrimination between self-compatible and self-incompatible cultivars.(6) S-allele genotypes of 16 Malus x domestica cultivars were identified by S-allele specific PCR analysis. It was suggested that no mutation occurred within a self-compatible cultivar 'Megumi'.

（1）结果表明，使用细胞器DNA的RFLP分析进行了3种Malus物种的细胞器DNA在玫瑰和苹果中的孕产妇遗传。细胞器DNA的数据。杂种与母亲父母形成了进化枝。在系统发育树上形成进化枝的某些物种之间存在表型差异，并且它们属于不同的部分。（4）证明RAPD分析是玫瑰品种和克隆植物识别的有用技术。（5）使用苹果中的S-RNase分析通过RFLP分析来鉴定Malus物种和品种。这些序列也可用于区分自兼容和自我兼容的品种。（6）通过S-Allele特定的PCR分析鉴定出16个Malus X家族品种的S替代基因型。有人提出，在自我兼容的品种“ Megumi”中不会发生突变。

项目成果

S.Matsumoto: "Characterization of phosphorylation sites in elongation factor 1β and β′" Sci. Rep. Fac. Educ., Gifu Univ. (Nat. Sci.). 19. 167-172 (1995)

S. Matsumoto：“延伸因子 1β 和 β 中磷酸化位点的表征”Sci.Rep.Fac.，Gifu Univ.（Nat.Sci.）19.167-172（1995）。

S.Matsumoto: "S-RNase like genomic sequences in apple for DNA fingerprinting" Acta Horticulturae. 392. 265-274 (1995)

S.Matsumoto：“苹果中用于 DNA 指纹识别的 S-RNase 样基因组序列”《园艺学报》。

S.Matsumoto and H.Fukui: "Identification of rose cultivars and clonal plants by random amplified polymorphic DNA." Scientia Horticulturae. 67. 49-54 (1996)

S.Matsumoto 和 H.Fukui：“通过随机扩增多态性 DNA 鉴定玫瑰品种和克隆植物。”

S. Matsumoto: "Molecular classification of wild roses using organelle DNA probes" Scientia Horticulturae. 印刷中.

S. Matsumoto：“使用细胞器 DNA 探针对野玫瑰进行分子分类”Scientia Horticulturae 正在出版。

脇田比呂美: "ミトコンドリアDNAのRFPL分析によるバラ野生種の分類・系統" 園芸学会雑誌. 64. 46-47 (1995)

Hiromi Wakita：“基于线粒体 DNA 的 RFPL 分析的野玫瑰物种的分类和系统学”园艺学会杂志 64. 46-47 (1995)。