Molecular phylogeny of Rasaceae based on S-RNase related and organelle DNA polymorphism

基于S-RNase相关和细胞器DNA多态性的蔷薇科分子系统发育

• 批准号: 07660032
• 负责人: MATSUMOTO Shogo
• 金额: $ 1.47万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660032/
• 关键词: Rose; Apple; organelle; self-incompatibility; Molecular taxonomy; RFLP; PCR

(1) The results demonstrate the maternal inheritance of organelle DNA in rose and apple.(2) Phylogenetic analysis among 19 Malus species were carried out using the RFLP analysis of organelle DNA.The molecular classification conformed closely to the traditional classification, but some species from one series belonged to different clades on phylogenetic trees.(3) Phylogenetic analysis among 17 wild rose species were carried out using the RFLP analysis and matK sequence data of organelle DNA.Interspecific hybrids formed a clade with maternal parents. There was a phenotypical difference between some species forming a clade on phylogenetic trees and they belonged to different sections.(4) It was demonstrated that RAPD analysis is a useful technique for rose cultivars and clonal plants identification.(5) Malus species and cultivars were identified by RFLP analysis using S-RNase like genomic sequences in apple. The sequences are also useful for discrimination between self-compatible and self-incompatible cultivars.(6) S-allele genotypes of 16 Malus x domestica cultivars were identified by S-allele specific PCR analysis. It was suggested that no mutation occurred within a self-compatible cultivar 'Megumi'.

(1)结果表明，玫瑰和苹果细胞器DNA具有母系遗传。(2)利用细胞器DNA的RFLP分析方法对19种苹果进行了系统发育分析。分子分类与传统分类基本一致，但在系统发育树上，同一系列的一些物种属于不同的分支。(3)利用RFLP分析和细胞器DNA的matK序列数据对17个野生玫瑰品种进行了系统发育分析。种间杂交种与母本形成一个分支。在系统发育树上，有些物种在形成一个分支时存在表型差异，它们属于不同的区段。(4) RAPD分析是一种有效的玫瑰品种和无性系鉴定技术。(5)利用苹果类似S-RNase的基因组序列，通过RFLP分析对苹果品种和栽培品种进行了鉴定。该序列还可用于自交亲和自交不亲和品种的区分。(6)采用s等位基因特异性PCR方法对16个海棠品种的s等位基因型进行了鉴定。自交亲和品种“Megumi”没有发生突变。

项目成果

S.Matsumoto: "S-RNase like genomic sequences in apple for DNA fingerprinting" Acta Horticulturae. 392. 265-274 (1995)

S.Matsumoto：“苹果中用于 DNA 指纹识别的 S-RNase 样基因组序列”《园艺学报》。

S.Matsumoto: "Characterization of phosphorylation sites in elongation factor 1β and β′" Sci. Rep. Fac. Educ., Gifu Univ. (Nat. Sci.). 19. 167-172 (1995)

S. Matsumoto：“延伸因子 1β 和 β 中磷酸化位点的表征”Sci.Rep.Fac.，Gifu Univ.（Nat.Sci.）19.167-172（1995）。

S. Matsumoto: "Molecular classification of wild roses using organelle DNA probes" Scientia Horticulturae. 印刷中.

S. Matsumoto：“使用细胞器 DNA 探针对野玫瑰进行分子分类”Scientia Horticulturae 正在出版。

S.Matsumoto and H.Fukui: "Identification of rose cultivars and clonal plants by random amplified polymorphic DNA." Scientia Horticulturae. 67. 49-54 (1996)

S.Matsumoto 和 H.Fukui：“通过随机扩增多态性 DNA 鉴定玫瑰品种和克隆植物。”

S.Matsumoto: "Characterization of phosphorylation sites in elongation factor 1beta and beta'." Sci.Rep.Fac.Educ., Gifu Univ. (Nat.Sci.). 19. 167-172 (1995)

S.Matsumoto：“延伸因子 1β 和 β 磷酸化位点的表征。”