Molecular phylogeny of Rosaceae based on S-allele genotype and organelle DNA polymorphism

基于S等位基因基因型和细胞器DNA多态性的蔷薇科分子系统发育

基本信息

批准号：
09660021
负责人：
MATSUMOTO Shogo
金额：
$ 1.86万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660021/
关键词：
Rose Apple Molecular taxonomy Self-incompatibility matK ITS S-allele PCR オルガネラ

项目摘要

(1) Phylogenetic analysis among 32 Rosa species was carried out using the matK sequence data of organelle DNA.The molecular classification conformed closely to the traditional classification with some anomalies such as polyphyly of section Pimpinellifoliae. As six original species that contributed to modern roses were found to belong to only one clade, the genetic background of modem roses seems to be a narrow one.(2) Phylogenetic analysis among 23 Rosa species was carried out using the ITS sequence data of nuclear DNA.The molecular classification was well in agreement with that of matK sequence analysis. Subgenus Platyrhodon and Hulthemia formed a clade within four subgenera.(3) S-allele genotypes of 46 Malus x domestica cultivars and Malus species were identified by S-allele specific PCR analysis. This analysis was useful in determining the genetic relationship within the genus Malus.(4) 'Tsugaru', 'Akagi', and 'Youkou' seem to have been produced by using pollen parents possessing the S_7-, S_7- or S_9-allele, respectively. It was suggested that there were at least three uncloned S-alleles. The S-genotypes of 'Kinsei' and 'Kizashi' were inconsistent with expected S-genotypes of their parents, indicating that they are derivatives from pollen parents having the S_9- or S_3-allele. A new S-allele, S_g, was proposed to be present in 'American Summer Pearmain', 'Indo', 'Kitanosachi', 'Shizuka', Mutsu' and 'Meku 10'.(5) A new S-RNase, S_g, shows a high similarity to SS_f (94%) at the deduced amino acid level. There are only two amino-acid differences between HV regions, which are responsible for the S-allele specificity of SS_g and SS_f.

（1）使用Organelle DNA的MATK序列数据进行了32种ROSA物种之间的系统发育分析。分子分类与传统分类与某些异常（例如Pimpinellifoliae段的多态）紧密符合传统分类。由于发现六个促成现代玫瑰的原始物种仅属于一个进化枝，因此，使用核DNA的序列数据进行了23种ROSA物种的调制解调器玫瑰的遗传背景。 Platyrhodon和Hulthemia子属在四个亚属内形成了一个进化枝。（3）通过S-Allele特定的PCR分析鉴定了46种Malus X horepera品种和Malus物种的S替代基因型。该分析对于确定马鲁斯属内的遗传关系很有用。（4）“ tsugaru”，“ akagi”和“ youkou”似乎是通过使用具有S_7-，s_7-或s_9-allele的花粉父母而产生的。有人提出至少有三个未串通的S- learter。 “ kinsei”和“ kizashi”的s型与父母的s基因型不一致，这表明他们是花粉父母的衍生物，这些父母的s_9-或s_3-allele。有人提出了一种新的S-Allele，S_G，以“美国夏季Pearmain”，“ Indo”，“ Kitanosachi”，“ Shizuka”，Mutsu'和Meku 10'中存在。（5）新的S-RNase，S_G，S_G在推定的Amino Amino酸水平上显示出与SS_F（94％）的高度相似之处。 HV区域之间只有两个氨基酸差异，这些差异负责SS_G和SS_F的S-Allele特异性。