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S-allele genotype of apple cultivars and establishment process for self-incompatibility

苹果品种S等位基因基因型及自交不亲和性建立过程

基本信息

批准号：
11660025
负责人：
MATSUMOTO Shogo
金额：
$ 2.43万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660025/
关键词：
Apple Self-incompatibility Malus S-locus S-allele PCR-RFLP Rosaceae Cross-incompatibility S遺伝子型バラ科植物 S-RNase PCR

项目摘要

( 1 ) The S-locus genes ( Sd-, Sh-, Se- and Si-RNase ) in the pistil were cloned and sequenced from the apple (Malus x domestica Borkh. ) cultivars. We developed an S-allele-specific polymerase chain reaction ( PCR )-restrictioon fragment length polymorphism ( RFLP ) analysis method for identification of the S-alleles. Using that method, we analyzed S-genotype of 136 apple cultivars and selections.( 2 ) The St- and Sg'-RNase CDNA were cloned from Malus trcmsitoria. S-allele genotypes of nine Malus species were identified by S-allele-specific PCR-RFLP analyzes.( 3 ) New S-RNases, Sg and Sh-RNase, and Sf-RNase show a strong similarity ( 93-94 % ) to each other at the deduced amino acid level. There are only two to six amino-acid differences among HV regions responsible for the S-allele specificity of Sf, Sg and Sh.( 4 ) Sg'-RNase differed by one amino acid from that of Sg-RNase. A difference of one amino acid within HV regions produced by natural mutation was not enough to generate a new S-allele. However, the model ofMaton et al. ( 1999 ) on establishing novel S-alleles was supported by one amino acid difference since S_g-allele could recognize both S_g- and S_g- alleles.( 5 ) Based on the phylogenetic analysis of S-RNases, it was speculated that the polymorphic S-RNases in apple were generated before divergence into the genera Malus and Pyrus.

(1)对苹果（Malus x domestica Borkh.）品种雌蕊s位点基因Sd-、Sh-、Se-和Si-RNase进行了克隆和测序。我们建立了s等位基因特异性聚合酶链反应(PCR)-限制性片段长度多态性（RFLP）分析方法来鉴定s等位基因。利用该方法对136个苹果品种及其选材进行了s基因型分析。(2)从苹果中克隆了St-和Sg'- rnase CDNA。采用s等位基因特异性PCR-RFLP方法对9种苹果的s等位基因型进行了鉴定。(3)新s - rnase、Sg - rnase和Sh-RNase以及Sf-RNase在推导出的氨基酸水平上具有很强的相似性（93 ~ 94%）。在负责Sf、Sg和Sh的s等位基因特异性的HV区域中，只有2 - 6个氨基酸的差异。(4)Sg'-RNase与Sg-RNase的差异为1个氨基酸。自然突变产生的HV区域内一个氨基酸的差异不足以产生一个新的s等位基因。然而，maton等人（1999）建立新的s等位基因的模型得到了一个氨基酸差异的支持，因为S_g等位基因可以同时识别S_g-和S_g-等位基因。(5)通过对s - rnase的系统发育分析，推测苹果中多态性s - rnase是在苹果属和梨属分化之前产生的。