Studies on molecular mechanisms underlying sex pheromone production in moths

飞蛾性信息素产生的分子机制研究

• 批准号: 17380041
• 负责人: MATSUMOTO Shogo
• 金额: $ 10.93万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380041/
• 关键词: silkmoth; pheromone; lipid droplet; lipolysis; G-protein coupled receptor; RNAi; PBAN receptor; acvl-CoA binding protein; 昆虫; 生合性酵素遺伝子; シグナル伝達; リン酸化; ESTデータベース; リパーゼ

The neurohormone PBAN (pheromone biosynthesis activating neuropeptide) regulates pheromone (bombykol) biosynthesis in the silkmoth, Bombyx marl, by binding to its cognate PBAN receptor, which is located in the pheromone gland (PG) cells of female moths. While extracellular Ca^<2+> had been shown to be essential for sex pheromone biosynthesis in all species studied to date, there had been no direct demonstration of this crucial event. To address this, we used fluorescent Ca^<2+> imaging techniques with isolated PGs to directly demonstrate that PBAN specifically triggers an influx of extracellular Ca^<2+>. Despite the efforts of numerous groups to understand the molecular mechanisms underlying bombykol production, little is known regarding how the external PBAN signal is converted to the calcium signal that drives bombykol production and release. To address this issue, we have taken advantage of the genome information in B. mori and characterized PG-specific genes[i.e. PG fatty acyl reductase (pgFAR), B. mori PG Z11/Δ10, 12 desaturase (Bmpgdesatl), PG acyl-CoA-binding protein (pgilCB1), midgut ACBP (mgACBI), and PBAN receptor (PBANR)] and successfully demonstrated their specific roles in bombykol biosynthesis in vivo by using an RNA interference-mediated loss-of-function approach.

神经激素PBAN（年级生物合成激活神经肽）通过与位于女雌性雌性腺体（pg）细胞中的丝绸pban受体结合，调节丝绸bombyx marl中的丝绸bombyx marl中的信息素（Bombykol）生物合成。虽然迄今为止，在所有研究的物种中，细胞外Ca^<2+>对于性别信息素生物合成至关重要，但尚无直接证明这一关键事件。为了解决这个问题，我们使用了带有孤立PG的荧光Ca^<2+>成像技术直接证明PBAN特异性触发了细胞外Ca^<2+>的影响。尽管众多小组为了解Bombykol生产的分子机制做出了努力，但对于如何将外部PBAN信号转换为驱动Bombykol生产和释放的钙信号，鲜为人知。为了解决这个问题，我们利用了B. mori中的基因组信息，并表征了PG特异性基因[即。 PG脂肪酰基还原酶（PGFAR），B。MORIPG Z11/δ10，12次饱和酶（BMPGDESATL），PG酰基酰基-COA结合蛋白（PGILCB1），Midgut ACBP（MGACBI）（MGACBI）和PBAN受体（PBANR）和PBAN受体（PBAN受体）以及成功的bom sefors in Compy in viv in Comes in Comes in Comes in Comes in Comes in Comes in Comes in Comes by in Comes byk in Comes in viv kens in viv in Comes in viv in Comes in viv kens in viv in Comes byk in con中RNA干扰介导的功能丧失方法。

项目成果

Determination of the pheromone producing region that has epoxidation activity in an abdominal tip of the Japanese giant looper, Ascotis selenaria cretacea (Lepidoptera : Geometridae)

日本大尺蠖腹尖具有环氧化活性的信息素产生区域的测定，Ascotis selenaria cretacea（鳞翅目：尺蛾科）

• 发表时间: 2007
• 期刊: J. Insect Physiol. 53
• 作者: Mise;K.;Furusawa;Takeda;Iwamoto;Takao;Iwahashi;Sasaki;Takeda;Watanabe;Tatsuta;Takeda;竹田篤史;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;松本正吾;Matsumoto S.;Shunya Takahashi;Takeshi Kawai;Takeshi Fujii
• 通讯作者: Takeshi Fujii

The Bombyx mori sex pheromone biosynthetic pathway is not mediated by cAMP

• DOI: 10.1016/j.jinsphys.2007.02.013
• 发表时间: 2007-08-01
• 期刊: JOURNAL OF INSECT PHYSIOLOGY
• 影响因子: 2.2
• 作者: Hull, J. Joe;Kajigaya, Ryosuke;Matsumoto, Shogo
• 通讯作者: Matsumoto, Shogo

Molecular mechanisms underlying sex pheromone production in the silkmoth,Bombyx mori

蚕蛾性信息素产生的分子机制

• 发表时间: 2007
• 期刊: J.Insect Physiol. 53
• 作者: Watanabe K.;Hull J. J.;Niimi T.;Imai K.;Matsumoto S.;Yaginuma T.;Kataoka H.;Shogo Matsumoto
• 通讯作者: Shogo Matsumoto

FXPRL-amide peptides induce ecdysteroidogenesis through a G-protein coupled receptor expressed in the prothoracic gland of Bombyx mori

• DOI: 10.1016/j.mce.2007.05.008
• 发表时间: 2007-07
• 期刊: Molecular and Cellular Endocrinology
• 影响因子: 4.1
• 作者: Ken Watanabe;J. Hull;T. Niimi;K. Imai;S. Matsumoto;T. Yaginuma;H. Kataoka

FXPRL-amide peptides induce ecdysteroidogensis through a G-protein coupled receptor expressed in the prothoracic gland of Bombyx mori

FXPRL-酰胺肽通过家蚕前胸腺中表达的 G 蛋白偶联受体诱导蜕皮类固醇生成

• 发表时间: 2007
• 期刊: Mol. Cell. Endocrinol. 273
• 作者: Mise;K.;Furusawa;Takeda;Iwamoto;Takao;Iwahashi;Sasaki;Takeda;Watanabe;Tatsuta;Takeda;竹田篤史;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;松本正吾;Matsumoto S.;Shunya Takahashi;Takeshi Kawai;Takeshi Fujii;Shogo Matsumoto;J. Joe Hull;Ken Watanabe
• 通讯作者: Ken Watanabe