Studies on molecular mechanisms underlying sex pheromone production in moths

飞蛾性信息素产生的分子机制研究

• 批准号: 17380041
• 负责人: MATSUMOTO Shogo
• 金额: $ 10.93万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380041/
• 关键词: silkmoth; pheromone; lipid droplet; lipolysis; G-protein coupled receptor; RNAi; PBAN receptor; acvl-CoA binding protein; 昆虫; 生合性酵素遺伝子; シグナル伝達; リン酸化; ESTデータベース; リパーゼ

The neurohormone PBAN (pheromone biosynthesis activating neuropeptide) regulates pheromone (bombykol) biosynthesis in the silkmoth, Bombyx marl, by binding to its cognate PBAN receptor, which is located in the pheromone gland (PG) cells of female moths. While extracellular Ca^<2+> had been shown to be essential for sex pheromone biosynthesis in all species studied to date, there had been no direct demonstration of this crucial event. To address this, we used fluorescent Ca^<2+> imaging techniques with isolated PGs to directly demonstrate that PBAN specifically triggers an influx of extracellular Ca^<2+>. Despite the efforts of numerous groups to understand the molecular mechanisms underlying bombykol production, little is known regarding how the external PBAN signal is converted to the calcium signal that drives bombykol production and release. To address this issue, we have taken advantage of the genome information in B. mori and characterized PG-specific genes[i.e. PG fatty acyl reductase (pgFAR), B. mori PG Z11/Δ10, 12 desaturase (Bmpgdesatl), PG acyl-CoA-binding protein (pgilCB1), midgut ACBP (mgACBI), and PBAN receptor (PBANR)] and successfully demonstrated their specific roles in bombykol biosynthesis in vivo by using an RNA interference-mediated loss-of-function approach.

神经激素PBAN（信息素生物合成激活神经肽）通过与位于雌蛾信息素腺（PG）细胞中的PBAN受体结合来调节蚕蛾信息素（Bombykol）的生物合成。虽然迄今为止，在所有研究过的物种中，细胞外Ca^2+都被证明是性信息素生物合成的关键，但还没有直接的证据证明这一关键事件。为了解决这个问题，我们使用荧光Ca^2+成像技术和分离的PG直接证明PBAN特异性地触发细胞外Ca^2+的流入。尽管许多研究小组努力了解家蚕酚产生的分子机制，但关于外部PBAN信号如何转化为驱动家蚕酚产生和释放的钙信号知之甚少。为了解决这个问题，我们利用了B中的基因组信息。mori和特征PG特异性基因[即PG脂肪酰基还原酶（pgFAR），B. mori PG Z11/Δ10，12去饱和酶（Bmpgdesatl）、PG酰基辅酶A结合蛋白（pgilCB 1）、中肠ACBP（mgACBI）和PBAN受体（PBANR）]的基因组序列，并通过RNA干扰介导的功能丧失方法成功地证明了它们在体内家蚕酚生物合成中的特异性作用。

项目成果

Further Studies of Lipid Droplets in the Bombykol‐Producing Pheromone Gland of Bombyx mori

• DOI: 10.1196/annals.1327.049
• 发表时间: 2005-04
• 期刊: Annals of the New York Academy of Sciences
• 影响因子: 5.2
• 作者: A. Fónagy;A. Ohnishi;Y. Esumi;Yoshikatsu Suzuki;S. Matsumoto

Determination of the pheromone producing region that has epoxidation activity in an abdominal tip of the Japanese giant looper, Ascotis selenaria cretacea (Lepidoptera : Geometridae)

日本大尺蠖腹尖具有环氧化活性的信息素产生区域的测定，Ascotis selenaria cretacea（鳞翅目：尺蛾科）

• 发表时间: 2007
• 期刊: J. Insect Physiol. 53
• 作者: Mise;K.;Furusawa;Takeda;Iwamoto;Takao;Iwahashi;Sasaki;Takeda;Watanabe;Tatsuta;Takeda;竹田篤史;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;松本正吾;Matsumoto S.;Shunya Takahashi;Takeshi Kawai;Takeshi Fujii
• 通讯作者: Takeshi Fujii

FXPRL-amide peptides induce ecdysteroidogenesis through a G-protein coupled receptor expressed in the prothoracic gland of Bombyx mori

• DOI: 10.1016/j.mce.2007.05.008
• 发表时间: 2007-07
• 期刊: Molecular and Cellular Endocrinology
• 影响因子: 4.1
• 作者: Ken Watanabe;J. Hull;T. Niimi;K. Imai;S. Matsumoto;T. Yaginuma;H. Kataoka

FXPRL-amide peptides induce ecdysteroidogensis through a G-protein coupled receptor expressed in the prothoracic gland of Bombyx mori

FXPRL-酰胺肽通过家蚕前胸腺中表达的 G 蛋白偶联受体诱导蜕皮类固醇生成

• 发表时间: 2007
• 期刊: Mol. Cell. Endocrinol. 273
• 作者: Mise;K.;Furusawa;Takeda;Iwamoto;Takao;Iwahashi;Sasaki;Takeda;Watanabe;Tatsuta;Takeda;竹田篤史;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;松本正吾;Matsumoto S.;Shunya Takahashi;Takeshi Kawai;Takeshi Fujii;Shogo Matsumoto;J. Joe Hull;Ken Watanabe
• 通讯作者: Ken Watanabe

神経ホルモンPBANが支配するカイコガ性フェロモン産生の分子機構

神经激素PBAN控制家蚕信息素产生的分子机制

• 发表时间: 2008
• 期刊: 化学と生物 46
• 作者: Mise;K.;Furusawa;Takeda;Iwamoto;Takao;Iwahashi;Sasaki;Takeda;Watanabe;Tatsuta;Takeda;竹田篤史;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;三瀬和之;松本正吾
• 通讯作者: 松本正吾