Research on Active Calcium Transport Systems of Plant Vacuoles

植物液泡活性钙转运系统的研究

基本信息

  • 批准号:
    07660106
  • 负责人:
  • 金额:
    $ 1.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1995
  • 资助国家:
    日本
  • 起止时间:
    1995 至 1996
  • 项目状态:
    已结题

项目摘要

In plant cells, vacuole is the main pool of calcium ions. The concentration of Ca^<2+> in the cytosol is kept at low levels, about a few hundred nanomolar. There are two different active Ca^<2+> transport systems on the plant vacuolar membranes. In this work, we established the assay method for the activity of Ca^<2+> uptake into the vacuolar membrane vesicles and determined the activities of Ca^<2+>-ATP ase and Ca^<2+>/H^+ antiporter individually. The Ca^<2+>/H^+ antiporter is driven by the proton gradient of the membrane vesicles generated by the vacuolar proton pumps. We determined the activity by a membrane filtration method with radioactive ^<45>Ca. Both activities were strongly inhibited by a calcium ionophore A23187. It means that ^<45>Ca was actively transported into the membrane vesicles. The Ca^<2+>-ATP ase and Ca^<2+>/H^+ antiporter exhibited different properties in the Km for Ca^<2+>. The Km values of Ca^<2+>-ATP ase and Ca^<2+>/H^+ antiporter for Ca^<2+> were about 2muM,and 20muM,respectively. The maximal activity of the antiporter was 3-times higher than the Ca^<2+>-ATP ase. From our observation, it was concluded that the Ca^<2+>-ATP ase is a high affinity, low capacity Ca^<2+> transporter, and that the Ca^<2+>/H^+ antiporter is a low affinity, high capacity Ca^<2+> transporter. It is estimated that a large quantity of cytoplasmic Ca^<2+> is taken up by Ca^<2+>/H^+ antiporter. The Ca^<2+>-ATP ase may reduce the cytoplasmic Ca^<2+> concentration less than a micromolar. Now we are sequencing the cDNAs for the Ca^<2+>/H^+ antiporter.
在植物细胞中,液泡是钙离子的主要贮存库。细胞质中Ca^2+的浓度保持在低水平,大约几百纳摩尔。植物液泡膜上存在两种不同的活性Ca^2+转运系统.本工作建立了液泡膜小泡Ca^2+摄取活性的测定方法,并分别测定了Ca^2+-ATP酶和Ca^2+/H^+逆向转运蛋白的活性。Ca^2+/H^+逆向转运蛋白由液泡质子泵产生的膜囊泡的质子梯度驱动。我们用放射性~(13)Ca通过膜过滤法测定活性<45>。这两种活性强烈抑制钙离子载体A23187。这意味着^<45>Ca被主动转运到膜囊泡中。Ca^2+-ATP酶和Ca^2+/H^+反向转运蛋白对Ca^2+的Km值表现出不同的性质。Ca^&lt;2+&gt;-ATP酶和Ca^&lt;2+&gt;/H^+逆向转运蛋白对Ca^&lt;2+&gt;的Km值分别约为2 μ M和20 μ M。反向转运蛋白的最大活性是Ca^&lt;2+&gt;-ATP酶的3倍。根据我们的观察,可以得出结论,Ca^2+-ATP酶是一种高亲和力、低容量的Ca^2+转运蛋白,而Ca^2+/H^+反向转运蛋白是一种低亲和力、高容量的Ca^2+转运蛋白。据估计,大量的胞质Ca^2+被Ca^2+/H^+逆向转运蛋白摄取。Ca^2+-ATP酶可使胞质Ca^2+浓度降低到1微摩尔以下。现在我们正在对Ca^2+/H^+反向转运蛋白的cDNA进行测序。

项目成果

期刊论文数量(33)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Matsumoto, H.: "Response of the plant root to aluminium stress: analysis of the inhibiton of the root elongation and chages in membrane function." Joumal of Plant Research. 109. 99-105 (1996)
Matsumoto, H.:“植物根部对铝胁迫的响应:根伸长抑制和膜功能变化的分析。”
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    0
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Maeshima,M.: "Proton pumps of vacuolar membrane in growing plant cell." Journal of Plant Research. 109. 119-125 (1996)
Maeshima,M.:“生长植物细胞中液泡膜的质子泵。”
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    0
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Matsumoto, H., Senoo, Y., Kasai, M., and Maeshima, M.: "Response of the plant root to aluminium stress : Analysis of the inhibition of the root elongation and changes in membrane function." J.Plant Res.109. 99-105 (1996)
Matsumoto, H.、Senoo, Y.、Kasai, M. 和 Maeshima, M.:“植物根部对铝胁迫的响应:根伸长抑制和膜功能变化的分析。”
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    0
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Odaira,M.: "Properties of the N-linked glycoproteins associated withnuclear envelope of mung bean hypocotyl." Plant and Cell Physiology. 36. 945-953 (1995)
Odaira,M.:“与绿豆下胚轴核膜相关的 N 连接糖蛋白的特性。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Maeshima, M.: "Proton pumps of vacuolar membrane in growing plant cell." Joumal of Plant Research. 109. 119-125 (1996)
Maeshima, M.:“生长植物细胞中液泡膜的质子泵。”
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MAESHIMA Masayoshi其他文献

MAESHIMA Masayoshi的其他文献

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{{ truncateString('MAESHIMA Masayoshi', 18)}}的其他基金

Molecular properties of signal transducing proteins on the plasma membrane
质膜上信号转导蛋白的分子特性
  • 批准号:
    25650093
  • 财政年份:
    2013
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Biochemical properties and physiological role of novel proteins involved in regulation of phosphatidylinositol phosphates.
参与磷脂酰肌醇磷酸调节的新型蛋白质的生化特性和生理作用。
  • 批准号:
    23657031
  • 财政年份:
    2011
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Molecular structures, functional coordination, and cell dynamics of vacuolar proton pump and metal transporters
液泡质子泵和金属转运蛋白的分子结构、功能协调和细胞动力学
  • 批准号:
    23248017
  • 财政年份:
    2011
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Molecular system of membrane transport and level regulation of metal ions
膜运输的分子系统和金属离子的水平调节
  • 批准号:
    20380060
  • 财政年份:
    2008
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular properties of metal-uptake systems supported by proton pumps in plant cells
植物细胞中质子泵支持的金属吸收系统的分子特性
  • 批准号:
    18380064
  • 财政年份:
    2006
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular structure and functional mechanism of a novel proton pump H+-pyrophosphatase
新型质子泵H-焦磷酸酶的分子结构及作用机制
  • 批准号:
    13142203
  • 财政年份:
    2001
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Study on inhibitors of vacuolar-type H^+-pyrophosphatase to search specific medicines for malaria disease
空泡型H^-焦磷酸酶抑制剂的研究寻找治疗疟疾的特效药物
  • 批准号:
    13556013
  • 财政年份:
    2001
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular diversities and structural construction, and intrecellular localization of plant aquqporins
植物水孔蛋白的分子多样性和结构构建以及细胞内定位
  • 批准号:
    13640645
  • 财政年份:
    2001
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular porperties and cellular function of aquaporins in the plasam and vacuolar membranes of plant
植物质体和液泡膜中水通道蛋白的分子特性和细胞功能
  • 批准号:
    11660084
  • 财政年份:
    1999
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular porperties and cellular function of aquaporins in the plasam and vacuolar membranes of plant
植物质体和液泡膜中水通道蛋白的分子特性和细胞功能
  • 批准号:
    09660114
  • 财政年份:
    1997
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Coordination of vacuolar proton-pumps during cold acclimation
冷驯化过程中液泡质子泵的协调
  • 批准号:
    317306136
  • 财政年份:
    2016
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    $ 1.41万
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Global significance of light-driven proton pumps in eukaryotic marine phytoplankton
光驱动质子泵在真核海洋浮游植物中的全球意义
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    NE/K013734/1
  • 财政年份:
    2013
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    $ 1.41万
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    Research Grant
Structure, function and dynamics of ATP synthases and rotary proton pumps
ATP 合成酶和旋转质子泵的结构、功能和动力学
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    nhmrc : 1022143
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    2012
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    $ 1.41万
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    Project Grants
Molecular mechanism of redox driven proton pumps
氧化还原驱动质子泵的分子机制
  • 批准号:
    7895528
  • 财政年份:
    2009
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    $ 1.41万
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Defining the role of the two vacuolar proton-pumps during stress responses
定义两个液泡质子泵在应激反应期间的作用
  • 批准号:
    76388002
  • 财政年份:
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Studies on the molecular evolution process of proton pumps by mutations of the multiheme-containing nitric oxide reductase
含多血红素一氧化氮还原酶突变的质子泵分子进化过程研究
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    19570125
  • 财政年份:
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    $ 1.41万
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    Grant-in-Aid for Scientific Research (C)
Molecular properties of metal-uptake systems supported by proton pumps in plant cells
植物细胞中质子泵支持的金属吸收系统的分子特性
  • 批准号:
    18380064
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通过固态核磁共振研究细菌质子泵 (P31)
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BIOENERGETICS AND PROTON PUMPS IN MALARIA PARASITES
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    7002735
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BIOENERGETICS AND PROTON PUMPS IN MALARIA PARASITES
疟疾寄生虫中的生物能量学和质子泵
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