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Studies on the biological activity of opines.

观点生物活性的研究。

基本信息

批准号：
07660262
负责人：
SATO Minoru
金额：
$ 1.6万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

It has been isolated five imino acids, such as octopine, tauropine, alanopine, strombine, and beta-alanopine, from marine invertebrates mainly marine molluscs. They have unique similar structure and are all referred to as "opines". Opines, which are biosynthesized by opine dehydrogenases, are now considered to be the end-products of anaerobic glycolysis of invertebrates. Opine dehydrogenases are found in almost all mollusks and many other invertebrates. Opines would be therefore ingested into the human body via foods, but the biological activities of opines are not yet known.The effects of dietary opines, octopine, tauropine and alanopine, on the level of serum and liver cholesterol of rats fed with cholesterol-enriched or cholesterol-free diets were investigated. Dietary supplementation with 1.5% octopine in a cholesterol-enriched diet significantly decreased the serum total-and VLDL+LDL-cholesterol levels and by contrary increased the serum HDL-cholesterol level in rats. The same tendency was observed in the rats fed with 1.5% octopine in a cholecterol-free diet. Contrary, dietary supplementation with 0.5% tauropine in a cholesterol-enriched diet significantly increased the serum total- and VLDL+LDL-cholesterol levels and decreased the serum HDL-cholesterol level in rats. Dietary supplementation with 1% alanopine in a cholesterol-enriched diet did not affect on the serum total cholesterol level. The cecum weight in the rats of opine groups ended also to be heavier than in that of the control rats. Most of rats fed tauropine and alanopine showed diarrhea.

已从海洋无脊椎动物（主要是海洋软体动物）中分离出五种亚氨基酸，例如章鱼碱、牛罗品、丙氨酸、斯特龙宾和β-丙氨酸。它们具有独特的相似结构，统称为“opines”。由冠松脱氢酶生物合成的冠松现在被认为是无脊椎动物无氧糖酵解的终产物。几乎所有软体动物和许多其他无脊椎动物中都含有 Opine 脱氢酶。因此，奥芬会通过食物摄入人体，但奥芬的生物活性尚不清楚。研究了膳食奥芬、章鱼碱、牛磺品和阿拉诺品对喂食富含胆固醇或不含胆固醇饮食的大鼠的血清和肝脏胆固醇水平的影响。在富含胆固醇的饮食中添加 1.5% 章鱼碱可显着降低大鼠血清总胆固醇和 VLDL+LDL 胆固醇水平，相反增加血清 HDL 胆固醇水平。在以不含胆固醇的饮食喂养 1.5% 章鱼碱的大鼠中也观察到了同样的趋势。相反，在富含胆固醇的饮食中添加0.5%牛磺酸会显着增加大鼠的血清总胆固醇和VLDL+LDL胆固醇水平，并降低血清HDL胆固醇水平。在富含胆固醇的饮食中添加 1% 阿拉诺平不会影响血清总胆固醇水平。结果，opine 组大鼠的盲肠重量也比对照组大鼠重。大多数喂食牛罗平和阿拉诺平的大鼠表现出腹泻。