The study for personal identification using DNA profiling method by organized bone marrows.

利用组织化骨髓 DNA 分析方法进行个人身份识别的研究。

• 批准号: 07670495
• 负责人: KUBO Shin-ichi
• 金额: $ 1.41万
• 依托单位: Nagasaki University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670495/
• 关键词: DNA profiling; PCR; personal identification; organized bone marrows; sex determination

We examined the organized human bone marrows with the aim of personal identification using PCR (the Polymerase Chain Reaction) analysis, that is sex determination and STR (short tandem repeats). The purpose of the present research is to disclose the optimum device to detection of DNA polymorphism with degraded DNA samples. The subjects were 38 manubrium sterni sampled at autopsies. We left them outdoor for periods from two weeks to 36 months, then DNA samples were extracted from bone marrows.1.We compared two primers of different bp length from amelogenin gene for sex determination. The sex identifications in 18 subjects were determinable with longer bp AMGL at first PCR analysis. Regarding to the other sex undeterminable cases, even then ten cases have still **en undeterminable although we took low molecules away and added BSA at PCR and moreover we tested Dual PCR.On the contrary, 25 ***aples were sex determinable with shorter bp AMGL at first PCR analysis. All cases have been sex de … More terminable when we performed PCRs when we removed low molecules form samples and added BSA.Thus we have arrived at the conclusion that followings are necessary to determine sexes with degraded DNA samples. Those are removal of low molecules and addition of BSA to the DNA samples Then designing shorter bp primer is preferable.2.We investigated STR types with vWF and TH01. Then we assessed the results whether or not those results would coincide between the samples providing from organized bone marrows and the blood DNA samples from each of the same individuals at autopsies. Thirteen samples were not amplified without preparation of DNA just the same as sex determination, but the products of all cases were clearly visible following the above preparation. The bands from organized bone marrow DNA and blood DNA at the time of autopsy coincided in all cases. Since both vWF and TH01 are shorter primers, when personal identification is demonstrated from organized DNA,it is necessary to increase the sensitivity of detection regarding to both DNA preparation and designing of the primers. Less

我们用PCR（聚合酶链式反应）分析，即性别测定和STR（短串联重复序列）检测了有组织的人骨髓，目的是进行个人鉴定。本研究的目的是揭示用降解DNA样品检测DNA多态性的最佳装置。研究对象为尸检中采集的38个胸骨柄。我们把它们放在室外，时间从两周到36个月不等，然后从骨髓中提取DNA样本。我们比较了两种不同bp长度的淀粉原蛋白基因引物的性别决定。其中18例在首次PCR分析中用较长bp AMGL进行了性别鉴定。对于其他无法确定性别的病例，尽管我们将低分子去除，在PCR中加入BSA，并进行了双PCR，但仍有10例病例无法确定。相反，25 ***个苹果在首次PCR分析中可以用较短的bp AMGL来确定性别。当我们从样品中去除低分子并添加BSA时，我们进行pcr时更容易确定。因此，我们得出的结论是，有必要用降解的DNA样本来确定性别。这两种方法分别是去除低分子和在DNA样品中加入牛血清蛋白，因此设计较短bp的引物是可取的。我们用vWF和TH01来研究STR类型。然后我们评估结果，这些结果是否与组织骨髓样本和同一个人尸检时的血液DNA样本相吻合。13个样本没有像性别决定一样在没有准备DNA的情况下进行扩增，但经过上述准备后，所有病例的产物都清晰可见。在所有病例中，尸检时骨髓DNA和血液DNA的条带一致。由于vWF和TH01都是较短的引物，当从有组织的DNA中进行个人鉴定时，需要从DNA制备和引物设计两方面提高检测灵敏度。少

项目成果

折原 義行: "陳旧骨髄からのAMXプライア-を用いたPCR法による性別判定" DNA多型. 3. 280-284 (1995)

Yoshiyuki Orihara：“利用 AMX 先验从旧骨髓中通过 PCR 方法确定性别”DNA 多态性。 3. 280-284 (1995)

折原義行: "陳旧骨髄からのAMXYプライマーを用いたPCR法による性別判定" DNA多型. 3. 280-284 (1995)

Yoshiyuki Orihara：“使用来自旧骨髓的 AMXY 引物通过 PCR 方法确定性别”DNA 多态性。 3. 280-284 (1995)

Orihara, Yoshiyuki: "Sex identification by PCR using AMXY Primer from Organized Bone Marrow" DNA Polymorphism. 3. 280-284 (1995)

Orihara, Yoshiyuki：“使用组织骨髓中的 AMXY 引物通过 PCR 进行性别鉴定”DNA 多态性。