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Immunohistochemical study on neuronal changes in brainstem nucleus of sudden infant death syndrome and various caouses of death

婴儿猝死综合征及各种死因脑干核神经元变化的免疫组织化学研究

基本信息

批准号：
09670442
负责人：
KUBO Shin-ichi
金额：
$ 2.11万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Scvcral nuclei in brain stem are well known to play an important role in supporting human life. However, the connection between neural changes of brain stem and the cause of death is not yet fully understood. To investigate the correlation of brain stem damage with the cause of death in forensic autopsy cases, neural changes of several nuclei were examined using immunohistochemical technique. Based in the cause of death, the forensic autopsy cases were divides into 6 groups as follows. Group A : hanging, ligature strangulation and manual strangulation, Group B : smothering arid choking, Group C : drowning, Group D : sudden infant death syndrome(SIDS), Group E : respiratory failure, Group F : heat stroke and sun stroke. The brain-stem were stained with the antibodies against microtuble-associated protein 2 (MAP2), muscalinic acetycholine receptor (mACh-R), c-fos gene product (c-Fos) and 72 kD heat-shock protein (HSP70), and the immunostaining of the-se markers in the arcuate nucleus (ARC), the hypoglossal nucleus (HN) and inferior olive (I0) was examined. In ease of asphyxia, neurons in HN were positively stained with both HSP70 and c-Fos antibodies, although morphological changes of these neurons was not evident. This may indicate that the occlusion of upper airway results in the damage to neurons in HN without their morphological changes. Positive staining of HSP70 and c-Fos in IO was more frequently observed in Group C than other groups. Since IO is involved in maintaining body balance which is often disturbed by drowning, it seems possible that neuronal damage in IO observed in drowning may be related to the disturbance of body balance. Consistent with the finding reported previously, the number of neurons with positive staining of mAChR in Group D was less than that in other groups. These observations indicate that immnuohistochemical study on the damage to neurons in brain stein nuclei can provide useful information for determining the cause of death.

脑干内的脊髓核在维持人类生命中起着重要的作用。然而，脑干神经变化与死亡原因之间的关系尚不完全清楚。应用免疫组化技术对法医尸检病例脑干损伤与死因的相关性进行了研究。根据死因，将法医尸检案件分为以下6组：A组：上吊、勒死和手勒死，B组：窒息和窒息，C组：溺水，D组：婴儿猝死综合征（SIDS）， E组：呼吸衰竭，F组：中暑和中暑。用抗微管相关蛋白2 （MAP2）、肌碱乙酰胆碱受体（mACh-R）、c-fos基因产物（c-fos）和72 kD热休克蛋白（HSP70）的抗体对脑干进行染色，并检测弓状核（ARC）、舌下核（HN）和下榄核（I0）中se标记物的免疫染色。在缺氧条件下，HSP70和c-Fos抗体对HN神经元均呈阳性染色，但形态学变化不明显。这可能表明上呼吸道闭塞导致HN神经元损伤，但其形态未发生改变。C组IO中HSP70和C - fos阳性染色较其他组多见。由于IO参与维持身体平衡，而身体平衡经常因溺水而受到干扰，因此溺水时观察到的IO神经元损伤似乎可能与身体平衡受到干扰有关。与之前报道的结果一致，D组mAChR染色阳性的神经元数量少于其他组。这些观察结果表明，免疫组织化学对脑stein核神经元损伤的研究可以为确定死亡原因提供有用的信息。