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Studies for the mechanism of adrenal hypertrophy induced by toluene inhalation, and endocrinological changes in adrenal gland

吸入甲苯所致肾上腺肥大机制及肾上腺内分泌变化的研究

基本信息

批准号：
11670418
负责人：
KUBO Shin-ichi
金额：
$ 1.92万
依托单位：
Department of Legal Medicine, School of Medicine, The university of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

The toluene is a principal ingredient of thinner that is the center of youth's abuse medicine. The toluene is well known to be high affinity for the brain and to present a various neurological and psychological symptoms. In this research, changing the adrenal by the toluene inhalation was examined. Wistar male rats were used. The rat was made to inhale toluene (1,000 ppm) for four hours a day. We examined for the inhalation group ; 4, 7 and 10 days of the inhalation against control group. As a result, the toluene inhalation induced adrenal hypertrophy, especially adrenal cortex. A significant difference was not admitted in serum ACTH level. However, it was suggested that the thalamus- pituitary - adrenal system related adrenal hypertrophy. It was forecast that the change in adrenal cortical internal secretion function took place based on adrenal cortical hypertrophy. However, the amount of mRNA of the adrenocortical hormone synthesis and serum aldosterone did not indicate significant. It was thought that the change stayed in an early temporary change even if there was hyper- synthesis and secretion of the hormone.Moreover, hyperexpression of GDNF, which was neurotrophic factor, was taken as changing adrenal medulla. We examined HSP70 and c-Fos in the neuron of spinal cord and in the chromaphine cell of adrenal medulla immunohistochemically. It was thought that the GDNF expression in adrenal medulla appeared due to the neuronal damage of spinal cord. This result suggested that toluene inhalation induce neuronal damage in spinal cord as a part of the CNS.

甲苯是稀释剂的主要成分，稀释剂是青少年滥用药物的中心。众所周知，甲苯对大脑有很高的亲和力，会引起各种神经和心理症状。在这项研究中，研究了吸入甲苯对肾上腺的改变。使用 Wistar 雄性大鼠。每天让大鼠吸入甲苯（1,000 ppm）四个小时。我们检查了吸入组；与对照组相比，吸入第4、7和10天。结果，吸入甲苯引起肾上腺肥大，尤其是肾上腺皮质肥大。血清ACTH水平未发现显着差异。但有人提出，肾上腺肥大与丘脑-垂体-肾上腺系统有关。据预测，肾上腺皮质分泌功能的改变是在肾上腺皮质肥大的基础上发生的。然而，肾上腺皮质激素合成和血清醛固酮的mRNA量并没有表明显着性。人们认为，即使存在激素的过度合成和分泌，这种变化也只是早期的暂时性变化。此外，GDNF（神经营养因子）的过度表达被认为是肾上腺髓质的变化。我们通过免疫组织化学方法检查了脊髓神经元和肾上腺髓质色素细胞中的 HSP70 和 c-Fos。人们认为肾上腺髓质中出现GDNF表达是由于脊髓神经元损伤所致。这一结果表明，吸入甲苯会导致中枢神经系统一部分的脊髓神经元损伤。