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EXPRESSION OF HEAT SHOCK PROTEIN ON HELICOBACTER PYLORI INFECTERD GASTRIC MUCOSA AND GASTRITIS

热休克蛋白在幽门螺杆菌感染者胃粘膜和胃炎中的表达

基本信息

批准号：
07670603
负责人：
SUGIYAMA Toshiro
金额：
$ 1.47万
依托单位：
SAPPORO MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670603/
关键词：
HELICOBACTER PYLORI HEAT SHOCK PROTEIN GASTRITIS

项目摘要

Helicobacter pylori (H.pylori) has causal role of histologic gastritis, gastro-duodenal ulcer and gastric carcinoma. The pathophysiology of histologic gastritis are closely associated with virulence factors of the organism and the host response to H.pylori. In this study, we have investigated the immune response of H.pylori infection. The expression of 66 kD protein was strongly induced in H.pylori after heat shock stimulation. The sequence analysis clearly demonstratedN-terminus of the 66kD protein was identical for HSP 60 family protein. By using rabbit anti-H.pylori HSP antibody, the expression of HSP protein was investigated in H.pylori infected gastric mucosa. Forty H.pylori positive histologic gastritis and 34 gastric ulcer tissues were studied. Eight of 40 gastritis tissues (20.0%) showedHSP expression on the epithelial cells. Eight of 34 gastric ulcer tissues (23.5%) had HSP expression on the epithelial cells. HSP is a cross-reactive protein between H.pylori organisms and H.pylori infected gastric epithelial cells. On the other hand, HSP antibody was detectedin serum of H.pylori infected patients. These results demonstratedthat immune response to HSP was induced in the patients. Although a infiltration of T cells was also observed, those cells had no expression or low expression of gamma/delta T cell receptor. It is required to investigate whether HSP is a target molecule recognized with intraepithelial cytotoxic T cells in H.pylori infected gastric mucosa.

幽门螺杆菌（Helicobacter pylori，H.pylori）是组织学胃炎、胃十二指肠溃疡和胃癌的致病菌。组织学胃炎的病理生理学与机体的毒力因子和宿主对幽门螺杆菌的反应密切相关。在这项研究中，我们研究了幽门螺杆菌感染的免疫反应。H.pylori经热休克刺激后，66 kD蛋白的表达被强烈诱导。序列分析表明，该蛋白的N端与HSP 60家族蛋白的N端完全相同。用兔抗H.pylori热休克蛋白（HSP）抗体检测H.pylori感染胃粘膜中HSP蛋白的表达。对40例H.pylori阳性胃炎和34例胃溃疡组织进行了研究。40例胃炎组织中8例（20.0%）上皮细胞有HSP表达。34例胃溃疡组织中8例（23.5%）上皮细胞有HSP表达。HSP是幽门螺杆菌与胃上皮细胞之间的交叉反应蛋白。H.pylori感染者血清中检测到HSP抗体。结果表明，患者体内存在对HSP的免疫应答。虽然也观察到T细胞浸润，但这些细胞不表达或低表达γ/δ T细胞受体。在幽门螺杆菌感染的胃粘膜中，HSP是否是上皮内细胞毒性T细胞识别的靶分子，有待进一步研究。