cDNA sequence of a 23kDa protein in human brain lesions.

为了确定与人类免疫缺陷病毒1型（HIV-1）基质蛋白（p17）抗体交叉反应的人蛋白，我们进行了蛋白纯化和cDNA克隆，获得了以下四个新发现1。抗体与一些人类蛋白质（不止一种蛋白质）发生交叉反应。通过硫酸铵切割、凝胶过滤、离子交换层析和二维凝胶电泳等方法纯化了人肝脏中的一种蛋白。n端氨基酸序列为KHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNV。该序列与人类超氧化物歧化酶-2 （SOD-2）的序列完全一致。人类SOD-2和HIV-1p17都含有六肽：LQPALK，这可能是一种共同的抗原决定因子。用上述抗体克隆人肝脏cDNA文库，我们检测到一个新的蛋白（399个氨基酸），该蛋白可能与内质网有关（与果蝇易位蛋白1的人类同源物）。通过克隆阿尔茨海默病海马的cDNA文库，我们检测到一个在n端有新序列的蛋白。该蛋白在c端也含有与嗜铬粒蛋白B相似的序列。

In order to determine human protein (s) which cross-react with antibodies against the matrix proein (p17) of human immunodeficiency virus type 1 (HIV-1), we perfomed protein purification from and cDNA cloning, and obtained four new findings below.1.The antibodies cross-reacted with some human proteins (more than one protein).2.One proein was purified to homogeneity from human liver by using ammonium sulphate cut, gel filtration, ion exchange chromatography, and two-dimensional gel electrophoresis. The N-terminal amino acid sequence was KHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNV.This sequence is completely consistent with that of human superoxide dismutase-2 (SOD-2). Both human SOD-2 and HIV-1p17 contain the hexapeptide : LQPALK which may serve as a common antigenic determinant.3.By human liver cDNA library cloning with one of the antibodies above, we detected a novel protein (399 amino acids) which seems to be associated with endoplasmic reticulum (a human homologue to Drosophila translocation protein 1).4.By cDNA library cloning from the hippocampus of Alzheimer's disease, we detected a protein which had a new sequence in the N-terninal portion. This protein also contained a sequence similar to that of chromogranin B in the C-terminal portion.