Studies on the intrauterine substance and mechanism that accelerates fetal lung maturation

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Chorioamnionitis (CAM) is considered as one of main causes of preterm delivery. It is reported that the fetal lung maturation of the premature infants delivered with CAM is accelerated compared with that of premature infants delivered without CAM.The parts of premature infants delivered with CAM are reported to diagnosed as having chronic lung disease including Wilson-Mikity syndrome. This chronic lung disease of premature infants exerts an influence on those prognoses. It is not clarified yet about the mechanism and substances that accelerate fetal lung maturation in CAM.Purposes of this study is to examine whether or not reactive oxygen species produced from the neutrophils that increase in amniotic fluid, involve as one of promotive substances of fetal lung maturation in CAM.We infused xanthine 1mM+xanthine oxidase (100mU/ml, 10mU/ml, 1mU/ml, 0.1mU/ml) system (X/XO) and produced reactive oxygen species in amniotic fluid in 16th day of pregnant wister rats and gather fetal lungs in the 19th day of gestation. We measured surfactant associated protein A,B,C (sp-A,B,C) mRNA in fetal lung. Sp-A,sp-B,sp-C mRNA of the group that administered X/XO obviously increased in comparison with that of control group of the 19th day of gestation by RT-PCR method. Also, we observed effects of allopurinol and catalase which attenuated the expression of sp-A,B,C mRNA.In conclusions, reactive oxygen species in amniotic fluid by X/XO administration were considered as one of the important factors which accelerate fetal lung maturation.