权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Functional capacity of the liver after partial hapatectomy in the rat

大鼠肝部分切除术后肝脏的功能能力

基本信息

批准号：
07671443
负责人：
HASHIMOTO Masaji
金额：
$ 0.38万
依托单位：
Okinaka Memorial Institute for Medical Reserch
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671443/
关键词：
functional liver mass partial hepatectomy functionla liver regeneration morphological liver regeneration indocyanine green aminopyrine epidermal growth factor somatostatin アンチピリン

项目摘要

Liver cell proliferation after partial hepatectomy in rats has been thoroughly investigated. Althorough DNA synthesis and morphological restoration has been studied in this rat model, functional capacity of the remnant liver during regeneration after hepatectomy has not been elucidated.We measured the ICG diaappearance rate (ICG-k) and serum aminopyrine and antipyrine clearance (CLamp, CLant) in rats at various intervals after 70% hepatectomy. ICG was measured with a photosensor (RK-1000) . Serum aminopyrine and antipyrine were measred by gas liqid chromatography. Morphological restoration of the liver after hepatectomy was evaluated based on remnant liver weight, the PCNA labeling index, and the DNA content of the regenerating liver. Serial changes in ICG-k, CLamp, and CLant after hepatectomy were compaired with the morhological restoration.ICG-k and CLamp were reduced by about one-third in 70% partial hepatectomy. Although restoration of remnantliver weight occurred steadily after the operation, ICG-k, CLamp, and CLant were lowest adout 36 hours after the operation. ICG-k was comparatively restored along with liver weight. The restoration of CLamp and Clant was delayd.Functional liver mass was minimal during parenchymal cell mitosis in the regenarating liver. Functional restoration after hepatectomy was delayd in comparison with morphological restoration.

对大鼠肝部分切除后的肝细胞增殖进行了深入的研究。大鼠肝切除后，残肝在再生过程中的功能尚不清楚。我们测定了大鼠肝切除后不同时间的ICG凋亡率(ICG-k)和血清氨基比林及安替比林清除率(CLAMP，CLant)。ICG用光电传感器(RK-1000)测量。血清氨基比林和安替比林用气相色谱法测定。根据残肝重量、增殖细胞核抗原标记指数和再生肝的DNA含量评价肝切除后肝脏的形态恢复情况。肝切除后ICG-k、CLAMP和CLANT的一系列变化与病理重建进行了比较。在70%的肝部分切除中，ICG-K和CLACK减少了约三分之一。虽然残肝重量在术后稳定恢复，但ICG-k、CLAMP和CLANT在术后36小时最低。ICG-k随着肝脏重量的增加而相对恢复。钳夹和Clant的恢复被推迟。在再生肝的实质细胞有丝分裂过程中，功能性肝质量最小。与形态恢复相比，肝切除后功能恢复延迟。