MORPHOLOGICAL AND FUNCTIONAL REGENERATION OF THE CIRRHOTIC LIVER AFTER PARTIAL HEPATECTOMY IN THE RAT

大鼠肝部分切除术后肝硬化的形态和功能再生

• 批准号: 09671352
• 负责人: HASHIMOTO Masaji
• 金额: $ 2.11万
• 依托单位: OKINAKA MEMORIAL INSTITUTE FOR MEDICAL RESEARCH
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671352/
• 关键词: liver cirrhosis; partial hepatectomy; functional capacity; functional regeneration; morphological regeneration; indocyanine green; aminopyrine; antipyrine; インドシアニングリーン

Background. Posthepatectomy liver insufficiency is one of the most serious problems associates with major hepatic resection especially in cirrhotic liver, which has less functional reserve than normal liver. It is important to evaluate morphological liver regeneration in cirrhotic rats as well as normal liver, and assessment of functional capacity in cirrhosis is clinically important for preventing insufficiency of the remnant liver after hepatectomy.Methods. We evaluated the functional capacity of the cirrhotic liver induced by carbon tetrachloride in rats, and studied the correlation between morphological and functional restoration of the liver after partial hepatectomy in comparison with normal rats. Morphological restoration of the liver after hepatectomy was evaluated on the basis of remnant liver weight, proliferating cell nuclear antigen labeling index, and the DNA content of the regenerating liver. Functional restoration was evaluated by the indocyanine green disappearance rate and serum aminopyrine clearance.Results. Morphological restoration in cirrhotic rats was delayed in comparison with normal rats. Although functional capacity in the cirrhotic rats was diminished, functional restoration after two-thirds hepatectomy was advanced in comparison with morphological restoration.Conclusions. Functional restoration in cirrhotic rats after partial hepatectomy is advanced in comparison with morphological restoration.

背景肝切除术后肝功能不全是肝切除术后最严重的问题之一，尤其是对于储备功能较正常肝脏差的肝硬化患者。重要的是要评估形态学肝再生在肝硬化大鼠以及正常的肝脏，并评估功能的能力，在肝硬化的临床上是重要的，以防止肝切除术后残肝功能不全。本实验观察了四氯化碳诱导的大鼠急性肝损伤后肝脏功能的变化，并与正常大鼠比较，探讨了肝部分切除后肝脏形态与功能恢复的相关性。根据残肝重量、增殖细胞核抗原标记指数和再生肝DNA含量评价肝切除术后肝脏的形态学恢复。以吲哚菁绿色消失率和血清氨基比林清除率评价功能恢复情况。与正常大鼠相比，中风大鼠的形态学恢复延迟。虽然肝硬化大鼠的功能能力下降，但与形态学恢复相比，三分之二肝切除术后的功能恢复是先进的。与形态学恢复相比，部分肝切除术后肝硬化大鼠的功能恢复是先进的。

项目成果

Minoru Okubo Masaji Hashimoto: "Glycogen storage disease III subtype and muscle meekness during childhood"Human Genetics. 104. 112-112 (1999)

Minoru Okubo Masaji Hashimoto：“糖原累积病 III 亚型和儿童时期的肌肉温顺”人类遗传学。

渡辺五朗、橋本雅司: "膵胃*合術-手技の手際"手術. 53(10). 1389-1394 (1999)

Goro Watanabe、Masashi Hashimoto：“胰胃手术 - 手术技术”53(10) (1999)。

Hashimoto M, Watanabe G.: "Functional capacity of the cirrhotic liver after partial hepatectomy in the rat"Surgery. 126(3). 541-547 (1999)

Hashimoto M，Watanabe G.：“大鼠部分肝切除术后肝硬化肝脏的功能”手术。

Masaji hashimoto, Goro Watanabe: "Simultances measurement of effective hepatic blood flow and systemic circulation"Hepatogastroenterology. (in press). (2000)

桥本正治、渡边五郎：“有效肝血流量和体循环的模拟测量”肝胃肠病学。

橋本雅司: "胆嚢良性疾患"超音波検査技術. 24(1). 17-24 (1999)

Masashi Hashimoto：“胆囊良性疾病”超声检查技术24（1）（1999）。