Effects of various drugs on pancreatic exocrine function in cerulein-induced acute pancreatitis in the rat

不同药物对雨蛙素诱发急性胰腺炎大鼠胰腺外分泌功能的影响

• 批准号: 07671425
• 负责人: SUGIYAMA Masanori
• 金额: $ 1.41万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671425/
• 关键词: acute pancreatitis; pancreatic exocrine function; cerulein; secretin

We experimentally investigated effect of secretin on pancreatic exocrine function in cerulein-induced acute pancreatitis in the rat. Cholecystokinin-stimulated pancreatic secretion was examined in conscious rats after surgical preparation with external pancreatic fistula. Four days after surgical preparation, we induced acute pancreatitis by intravenous administration of cerulein at a rate of 10 mug/kg/h for 4 hours, followed by administration of normal saline (control group) or secretin (5CU/kg/h, 8 hours ; secretin group). Pancreatic exocrine function test was undertaken one day before and after cerulein administration. Microscopically, the pancreas in control group showed prominent interstitial edema and intracellular vacuolization of the acinar cell. These findings were partially improved after secretin administration. In control group, basal and cholecystokinin-stimulated pancreatic juice flow, bicarbonate output, and protein output decreased significantly after cerulein administration. Secretin reestablished pancreatic juice flow and bicarbonate output, and partially restored protein output. In rats with cerulein-induced pancreatitis, secretin reduced pancreatic histopathology and restored exocrine dysfunction, when compared with non-secretin-treated rats.

本实验观察促胰液素对蛙皮素诱导的大鼠急性胰腺炎胰腺外分泌功能的影响。在清醒大鼠胰外瘘手术准备后，检测了胆囊收缩素刺激的胰腺分泌。手术准备后4天，我们通过以10 μ g/kg/h的速率静脉内给予雨蛙肽4小时，随后给予生理盐水（对照组）或促胰液素（5CU/kg/h，8小时;促胰液素组）诱导急性胰腺炎。在雨蛙肽给药前和给药后1天进行胰腺外分泌功能检测。镜下对照组胰腺间质水肿明显，腺泡细胞内空泡化。这些结果在促胰液素给药后部分改善。在对照组中，基础和胆囊收缩素刺激的胰液流量，碳酸氢盐输出量和蛋白输出量在雨蛙肽给药后显著降低。促胰液素重建了胰液流量和碳酸氢盐输出，并部分恢复了蛋白质输出。在雨蛙素诱导的胰腺炎大鼠，胰泌素减少胰腺组织病理学和恢复外分泌功能障碍，与非胰泌素治疗的大鼠相比。