Immunohistochemical localization of beta1,4-galactosyltransferase in human normal and neoplastic pancreatic tissues

beta1,4 -半乳糖基转移酶（GalTase）是高尔基体中的糖基转移酶，它将半乳糖从udp -半乳糖转移到糖缀合物末端的n -乙酰氨基葡萄糖残基上，形成beta1,4键。肿瘤自身糖基转移酶的碳水化合物部分发生各种变化。这一过程也表明糖基转移酶本身可能发生变化。因此，我们检测了一种单克隆抗体（MAb8628）在正常和肿瘤外分泌组织中对抗GalTase的结合模式。10个正常和II肿瘤人外分泌胰腺组织从外科手术获得。冷冻切片用该抗体孵育。正常导管细胞和腺泡细胞的核上区和末端条光镜下显示GalTase阳性。中央腺泡细胞笔核区呈阳性染色。肿瘤细胞的核上区和终末条也被染色。与正常细胞相比，肿瘤细胞核上区发育良好，染色强烈。超核区和末端条分别对应于高尔基体的反池和连接复合物（即紧密连接和粘附连接），在电子显微镜水平上可见。胰腺肿瘤改变导致高尔基体中GalTase表达增加，其增加可能对胰腺上皮细胞间的粘附和通讯有重要影响。这种酶的测定有助于诊断正常组织外表皮胰腺肿瘤的变化。

beta1, 4-galactosyltransferase (GalTase) is the glycosyltransferase in the Golgi apparatus that transfers galactose from UDP-galactose to terminal N-acetylglucosamine residues in glycoconjugates with formation of beta1,4 linkage. Neoplasms undergo various changes in the carbohydrate moieties of their glycosyltransferase themselves. This process also indicates the possibility of changes in glycosyltransferase themselves. Therefore, we examined the binding pattern of a monoclonal antibody (MAb8628) against GalTase in both normal and neoplastic exocrine tissues. Ten normal and II neoplastic human exocrine pancreatic tissues obtained from surgical operations were used. Frozen sections were incubated with this antibody. Supra-nuclear regions and terminal bars of normal duct cells and acinar cells revealed positive staining for GalTase at the light microscopic level. Centroacinar cells revealed positive staining in their pen-nuclear region. Neoplastic cells were also stained in their supra-nuclear regions and terminal bars. Supra-nuclear regions were well developed in neoplastic cells and intensely stained compared with those in normal cells. The supra-nuclear regions and the terminal bars corresponded to the trans cistern of the Golgi apparatus and the junctional complex (i. e. , tight junction and adherens junction), respectively, seen at the electron microscopic level. Pancreatic neoplastic changes thus lead to an increase in the expression of GalTase in the Golgi apparatus, which increase may have an important effect on the intercelluar adhesion and communication among pancreatic epithelial cells. Measurement of this enzyme is useful for diagnois of exocnine pancreatic neoplastic change from normal tissues.