Immunohistochemical localization of beta1,4-galactosyltransferase in human normal and neoplastic pancreatic tissues

β 1，4-半乳糖基转移酶（GalTase）是高尔基体中的糖基转移酶，其将半乳糖从UDP-半乳糖转移到糖缀合物中的末端N-乙酰葡糖胺残基，形成β 1，4键。新霉素在其糖基转移酶自身的碳水化合物部分中经历各种变化。这一过程也表明糖基转移酶本身可能发生变化。因此，我们研究了正常和肿瘤外分泌组织中的单克隆抗体（MAb 8628）对GalTase的结合模式。10个正常和II肿瘤的人外分泌胰腺组织从外科手术中获得。将冷冻切片与该抗体孵育。光镜下，正常导管细胞和腺泡细胞的核上区和终末条呈GalTase阳性染色。中央腺泡细胞在其笔核区域显示阳性染色。肿瘤细胞的核上区域和终末条也被染色。与正常细胞相比，肿瘤细胞的核上区发达，染色较强。核上区和终杆分别对应于高尔基体的跨池和连接复合体。e.紧密连接和粘附连接）。因此，胰腺肿瘤性变化导致高尔基体中GalTase表达增加，其增加可能对胰腺上皮细胞之间的细胞间粘附和通讯具有重要影响。测定这种酶有助于诊断胰腺癌的发生。

beta1, 4-galactosyltransferase (GalTase) is the glycosyltransferase in the Golgi apparatus that transfers galactose from UDP-galactose to terminal N-acetylglucosamine residues in glycoconjugates with formation of beta1,4 linkage. Neoplasms undergo various changes in the carbohydrate moieties of their glycosyltransferase themselves. This process also indicates the possibility of changes in glycosyltransferase themselves. Therefore, we examined the binding pattern of a monoclonal antibody (MAb8628) against GalTase in both normal and neoplastic exocrine tissues. Ten normal and II neoplastic human exocrine pancreatic tissues obtained from surgical operations were used. Frozen sections were incubated with this antibody. Supra-nuclear regions and terminal bars of normal duct cells and acinar cells revealed positive staining for GalTase at the light microscopic level. Centroacinar cells revealed positive staining in their pen-nuclear region. Neoplastic cells were also stained in their supra-nuclear regions and terminal bars. Supra-nuclear regions were well developed in neoplastic cells and intensely stained compared with those in normal cells. The supra-nuclear regions and the terminal bars corresponded to the trans cistern of the Golgi apparatus and the junctional complex (i. e. , tight junction and adherens junction), respectively, seen at the electron microscopic level. Pancreatic neoplastic changes thus lead to an increase in the expression of GalTase in the Golgi apparatus, which increase may have an important effect on the intercelluar adhesion and communication among pancreatic epithelial cells. Measurement of this enzyme is useful for diagnois of exocnine pancreatic neoplastic change from normal tissues.