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Opsonin Receptor Expression on Peritoneal Exudative and Circulatory Neutrophils in Murine Acute Pancreatitis

小鼠急性胰腺炎腹膜渗出液和循环中性粒细胞调理素受体的表达

基本信息

批准号：
13671335
负责人：
SUGIYAMA Masanori
金额：
$ 1.98万
依托单位：
Kyorin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671335/
关键词：
Acute pancreatitis Bacterial infection Opsonin receptor Opsonin Receptor G-CSF

项目摘要

Acute severe (necrotizing) pancreatitis is often associated with pancreatic or peripancreatic infection. Decreased bacterial clearance due to impaired immune defense may cause local infection. We investigated expressions of surface opsonin receptors (CD11b, complement receptor 3 ; CD32/CD16, immunoglobulin G Fc receptor) on local and circulatory neutrophils, in murine acute pancreatitis. The mild and severe forms of acute pancreatitis were induced by 7 and 13 subcutaneous injections of caerulein, respectively. Peritoneal exudative and circulatory neutrophils were counted and assayed for receptor expressions by flow cytometry, serially at 1-72 hours after pancreatitis induction. Histologically, mild and severe forms showed edematous and necrotizing pancreatitis, respectively. The peritoneal exudative neutrophil count was greater in mild than in severe pancreatitis. Expressions of CD11b and CD32/CD16 on local neutrophils were upregulated early in mild pancreatitis. This upregulation was attenuated in severe pancreatitis. The circulatory neutrophil count was elevated in severe pancreatitis but was unchanged in mild pancreatitis. Opsonin receptor expression on circulatory neutrophils showed a transient, modest upregulation in the early phase of mild pancreatitis. Receptor-positive circulatory neutrophils showed a marked elevation which persisted throughout the course of severe pancreatitis. In conclusion, severe (necrotizing) pancreatitis is associated with reduced opsonin receptor expression on local neutrophils and enhanced expression on circulatory neutrophils, as compared with mild (edematous) pancreatitis. These changes may contribute to local infectious complications and multiple organ failure, in severe pancreatitis.

急性重症（坏死性）胰腺炎通常与胰腺或胰周感染有关。由于免疫防御受损而导致的细菌清除率降低可能导致局部感染。我们研究了小鼠急性胰腺炎局部和循环中性粒细胞表面调理素受体（CD 11b，补体受体3 ; CD 32/CD 16，免疫球蛋白G Fc受体）的表达。分别皮下注射雨蛙肽7次和13次，诱发轻度和重度急性胰腺炎。在胰腺炎诱导后1-72小时连续计数腹膜渗出和循环中性粒细胞，并通过流式细胞术测定受体表达。组织学上，轻度和重度形式分别表现为水肿性和坏死性胰腺炎。腹膜渗出性中性粒细胞计数轻度胰腺炎高于重度胰腺炎。轻症胰腺炎早期局部中性粒细胞CD 11b和CD 32/CD 16表达上调。这种上调在重症胰腺炎中减弱。循环中性粒细胞计数在重症胰腺炎中升高，但在轻度胰腺炎中无变化。在轻度胰腺炎的早期，循环中性粒细胞上的调理素受体表达表现出短暂的、适度的上调。受体阳性的循环中性粒细胞显示出显着的升高，持续整个过程中的重症胰腺炎。总之，与轻度（水肿性）胰腺炎相比，重度（坏死性）胰腺炎与局部中性粒细胞上调理素受体表达减少和循环中性粒细胞上调理素受体表达增强有关。这些变化可能会导致局部感染并发症和多器官功能衰竭，在重症胰腺炎。