Mechanism and prophyaxis in post traumatic epilepsy-in vivo study on free radicals in iron-indced epileptogenic foci formation

将铁溶液注入大鼠感觉运动皮层诱发致痫性放电，该模型被认为是创伤后癫痫。许多体外实验表明，羟基自由基是铁诱发癫痫发作的触发因素。本研究采用脑电描记法记录大鼠纹状体内注射铁溶液诱发癫痫放电，并采用L-波段电子自旋共振计算机断层扫描（EST-CT）技术，在大鼠纹状体内注射铁溶液1小时后，腹腔内注射氮氧自由基自旋标记物氨甲酰-PROXYL，获得纹状体内氮氧自由基图像。在此过程中，我们获得了一些在体数据，为大鼠脑的氮氧自由基成像提供了补充：（1）L-带电子自旋共振（ESR）结果表明，大鼠感觉运动皮层注射铁溶液后1小时，腹腔注射氨甲酰-PROXYL可延长大鼠头部氮氧自由基信号的衰减。 ...更多信息 nance光谱仪和自由基清除剂的管理，日本草药Shosaikoto-go-Keishi-ka-Shakuyaku-to抑制延迟的氮氧自由基信号衰减。(2)由于在注射氨甲酰-PROXYL后，使用L-波段ESR-CT将铁溶液注入感觉运动皮层后，难以获得大鼠皮层中的氮氧自由基图像，因此我们尝试在将铁溶液注入大鼠纹状体后诱发癫痫放电，并通过EEG记录发现在注射铁溶液后1周出现癫痫放电。(3)腹腔注射氨甲酰基-PROXYL后，L-带ESR-CT显示纹状体内注入铁溶液后1h内有少量的氮氧自由基分布，从注入铁溶液后3 h至1d，氮氧自由基面积逐渐增大。纹状体内一氧化氮自由基的分布在注射后1周较注射后1天明显增大。这些图像数据表明，它是能够找到组织损伤的区域，在大脑中使用L波段ESR-CT方法。此外，儿茶素混合溶液，唑尼沙胺，氨基胍，草药提取物的Toki-shakyakusan和罗汉果提取物抑制脂质过氧化物的形成后，大鼠的大脑皮层注射铁溶液到感觉运动皮层，这些抗氧化剂可能是一种预防铁诱导的癫痫形成。少

The injection of iron solution into the sensory motor cortex of rats induces epileptogenic discharges and this model is regarded as a post traumatic epilepsy. Many in vitro experiments suggest that hydroxyl radical is a trigger for iron-induced epileptogenic seizures. In the present study we established to induce epileptogenic discharges after injection of iron solution into the striatm of rats using EEG recording and obtained nirtoxide radicals images in the striatum one hour afer injection of iron solution into the striatum of rats with intraperitioneal injection of nitroxide spin label, carbamoyl-PROXYL by L-band electron spin resonance-computed tomography (EST-CT). During this proacess we got some in vivo data to add for nitroxide imaging of rat brain as follows ; (1) The decay of nitroxide radical signals in rat head one hour after iron injection of iron solution into the sensory motor cortex prolonged with intraperitoneal injection of carbamoyl-PROXYL by L-band electron spin reso … More nance spectrometer and the administration of free radical scavenger, Japanese herbs of Shosaikoto-go-Keishi-ka-Shakuyaku-to inhibited the delay of the nitroxide radical signal decay. (2) As it was hard to obtain nitroxide radical images in the cortex of rats after indection of iron solution into the sensory motor cortex using L-band ESR-CT after injection of carbamoy-PROXYL,we tried to induce epileptogenic discharges after injection of iron solution into the striatum of rats and we found the appearance of epileptogenic discharges one week after injection of it by EEG recording. (3) The slight distribution of nitroxide radicals were observed in the striatum one hour after injection of iron solution into the striatum by L-band ESR-CT after intraperitioneal injection of carbamoyl-PROXYL and the area of nitroxide radicals became gradually enlarge from 3 hours to one day after injection of iron solution. The size of nitroxide radical distribtion in the striatum became enlage more one week after injection compared with that of one day after injection. These images data show that it is able to find tissue damaged areas in the brain using L-band ESR-CT method. In addition, catechin mix solution, zonisamide, aminoguanidin, herbal extract of Toki-shakyakusan and frucutus Momordicae extract inhibited the lipid peroxide formation in the cerebral cortex of rats after injection of iron solution into the sensory motor cortex, and these antioxidants may be a prophylactic for iron-induced epileptogenic formation. Less