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The study of inflammatory and defensive mechanisms in otitis media with effusion.

渗出性中耳炎炎症和防御机制的研究。

基本信息

批准号：
07671872
负责人：
HIMI Tetsuo
金额：
$ 1.41万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671872/
关键词：
Otitis media Cytokine Chemokine Neutrophil Adhesion molecules 走化因子

项目摘要

In this project, in vivo induction and regulation of chemokine, cytokines and cell adhsion molecules in the middle ear were investigated using topical inoculation with KLH,lopopolysaccharide (LPS) and Lipoteichoic acid (LTA) in rat model. The expressions of mRNA in the middle ear mucosa was also examined.At first, we examined the expression of cell adhesion molecules (CAM) in immune-mediated otitis media in the rat, as well as the regulation of these CAM in peripheral blood polymorphonuclear leukocytes (PMN) and lymphocytes upon exposure to middle ear effusion (MEE). The expression of CAM was important for the initiation of otitis media. Moreover, it ws thought that the interaction between the infiltrated PMN and MEE may modify the CAM's expression during the inflammatory process in the middle ear cavity.Chemokine possesses chemotactic-activating properties toward neutrophils, and may contribute to the pathogenesis of middle ear inflammations. We investigated the production of chemokine in middle ear lavage and that gene expression in the middle mucosa using topical inoculation with LPS and LTA in the rat in vivo model. In contrtast to LPS as gram-negative endotoxin, lipoteichoic acid (LTA) is present within this layr of LPS as gram-negative endotoxin, lipoteichoic acid (LTA) is present within this layr of mo gram-positive bacteria. LTA has been shown to play a sigificant role in the initiation and progression of bacterial infection. Chemokine in middle ear lavage showed time-and dose-dependent production under LPS or LTA stimulation. At the time of peak production, the expression of cytokines and chemokine mRNA,evaluated using polymerase chain reaction, was considerable in the middle ear mucosa.This investigation of the characteristics of proinflammatory cytokines in the middle ear cavity using rat in vivo model has extended the functional concept of cytokines at the initial stage otitis media.

本课题通过局部接种KLH、脂多糖（LPS）和脂磷壁酸（LTA），观察了在体诱导和调节中耳内趋化因子、细胞因子和细胞粘附分子的作用。首先，我们观察了免疫介导的中耳炎大鼠模型中细胞粘附分子（CAM）的表达，以及中耳积液（中耳积液）对外周血中性粒细胞（PMN）和淋巴细胞中CAM表达的调节。CAM的表达在中耳炎的发生中起重要作用。此外，在中耳炎的发生发展过程中，中性粒细胞与趋化因子之间的相互作用可能改变了CAM的表达，趋化因子对中性粒细胞具有趋化激活作用，可能参与了中耳炎的发生发展。我们在大鼠体内模型中，采用局部接种LPS和LTA的方法，研究了中耳灌洗液中趋化因子的产生和中粘膜中趋化因子的基因表达。脂磷壁酸（LTA）是革兰氏阴性细菌的内毒素，脂磷壁酸（LTA）是革兰氏阳性细菌的内毒素。LTA在细菌感染的发生和发展中起重要作用。在LPS或LTA刺激下，中耳灌洗液中的趋化因子显示时间和剂量依赖性的产生。在高峰生产的时候，细胞因子和趋化因子mRNA的表达，使用聚合酶链反应评价，是相当大的中耳mucosa.This调查的特点，促炎细胞因子在中耳腔中使用大鼠在体内模型扩展了功能的概念，细胞因子在中耳炎的初始阶段。