The study of a relationship between innate immunity and child recurrent infection in upper respiratory airway

天然免疫与儿童上呼吸道反复感染关系的研究

• 批准号: 12671676
• 负责人: HIMI Tetsuo
• 金额: $ 2.43万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671676/
• 关键词: Innate Immunity; Infectious disease; Drug resistance; TLR; signal transduction; 生体防御機構; サイトカイン; 細菌抗原; 鼻咽腔

The inflammatory mediators are intimately involved in the inflammatory cascade occurring in the upper air way and middle ear. Recent evidence reveals that innate immunity has an important role to play in the initial defense mechanisms. The innate immune system identifies bacteria by means of a pattern-recognition receptor. Mammalian Toll-like receptors (TLRs) are considered to be key receptors in this system. Stimulation of target cells with bacterial component results in a TLR-dependent the activation of NF kappa B leading to the production of pro-inflammatory cytokines. In this study, the production of cytokines, induced by representative pathogens and their components, was studied using not only animal model but also effector cell lines. The relationship between pathogens and TLR2 was also elucidated. Moreover, we referred to a new species of middle ear pathogen, Alloiococcus otitidis.

炎症介质密切参与上气道和中耳中发生的炎症级联反应。最近的证据表明，先天免疫在最初的防御机制中发挥着重要作用。先天免疫系统通过模式识别受体来识别细菌。哺乳动物Toll样受体（TLR）被认为是该系统中的关键受体。用细菌组分刺激靶细胞导致TLR依赖性NF κ B活化，导致促炎细胞因子的产生。在这项研究中，细胞因子的生产，诱导的代表性病原体及其成分，不仅使用动物模型，而且效应细胞系进行了研究。阐明了病原菌与TLR2的关系。此外，我们提到了一种新的中耳病原菌，耳异球菌。

项目成果

Himi T, Kita H, Mitsuzawa H, Harimaya A, Tarkkanen J, Hendolin P, Ylikoski J, Fujii N: "Effect of Alloiococcus otitidis and three pathogens of otitis media in production of interleukin-12 by human monocyte cell line"FEMS Immunol Med Microbiol. 29(2). 101-

Himi T、Kita H、Mitsuzawa H、Harimaya A、Tarkkanen J、Hendolin P、Ylikoski J、Fujii N：“中耳异球菌和三种中耳炎病原体对人单核细胞系产生白细胞介素 12 的影响”FEMS 免疫学医学

Himi T, et al: "Effect of Alloiococcus otitidis and three pathogens of otitis media in production of IL-12 by human monocyte cell line"FEMS Immunol Med Microbiol. 29(2). 101-106 (2000)

Himi T 等人：“耳炎异球菌和三种中耳炎病原体对人单核细胞系产生 IL-12 的影响”FEMS 免疫医学微生物学。

Tarkkanen J, Himi T. Atshushi H, Carlson P, Ylikoski J, Mattila PS: "Stimulation of adenoidal lymphocytes by Alloiococcus otitidis"Ann Otol Rhinol Laryngol. 109(10 Pt1). 958-964 (2000)

Tarkkanen J、Himi T. Atshushi H、Carlson P、Ylikoski J、Mattila PS：“耳炎异球菌对腺样体淋巴细胞的刺激”Ann Otol Rhinol Laryngol。

Kita H, Himi T, Fujii N, Ylikoski J: "Interleukin-8 secretion of human epithelial and monocytic cell lines induced by middle ear pathogens"Microbiol Immunol. 44(6). 511-517 (2000)

Kita H、Himi T、Fujii N、Ylikoski J：“中耳病原体诱导的人上皮细胞和单核细胞系的白细胞介素 8 分泌”微生物免疫学。

Mitsuzawa, H. et al.: "Extracellular toll-like receptor 2 region containing serine4O-Isoleucine64 but not cysteine3O-serine39 is critical for the recognition of Staphylococcus aureus peptidoglycan"J. Biol. Chem. 276. 41350-41356 (2001)

Mitsuzawa, H. 等人：“含有丝氨酸 4O-异亮氨酸 64 但不含有半胱氨酸 3O-丝氨酸 39 的细胞外 Toll 样受体 2 区域对于识别金黄色葡萄球菌肽聚糖至关重要”。