The Chemical Analysis and Function of "Mekabu" (Sporophyll of Undaria pinnatifida) Element

裙带菜“Mekabu”元素的化学分析及功能

• 批准号: 07680049
• 负责人: OGAWA Noriko
• 金额: $ 0.9万
• 依托单位: Gifu Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680049/
• 关键词: "Mekabu"; alginic acid; viscosity; fermentation; short-chain fatty acids; plasma cholesterol; cecum; 小腸

"Mekabu" is the sporophyll formed near the root of "Wakame" (undaria pinnatifida). The viscous exudate of the "Mekabu" mainly consists of alginic acid. The aim of this study was to clarify the physiological effects and chemistry of "Mekabu".The viscosity and alginic acid content of viscous exudate extracted from "Mekabu" were compared with "Wakame" and "Konbu". The viscosity was determined with an E type viscometer and the alginic acid content was analyzed by HPLC.The viscosity and alginic acid content of viscous exudate extracted from "Mekabu" were greater than those of exudate extracted from "Wakame" or "Konbu".Indigestible polysaccharides can be fermented in the large intestine to produce short-chain fatty acids (SCFA). Almost all of SCFA are absorbed and enter the systemic energy pool of the host animal. Apart from their energy contribution, SCFA have physiological influences on digestive organs. Accordingly, we measured released gas and SCFA production from sodium alginate or viscous exudate extracted from "Mekabu" in batch culture using pig cecal bacteria. The negligible gas release and SCFA production from cultures suggested that alginic acid was poorly fermentable for hindgut bacteria.The addition of viscous dietary fiber to the diet causes an enlargement of digestive organs. The viscosity of dietary fiber may play an important role in these effects. We observed influences of "Mekabu" on plasma cholesterol, digestive organs and cecal contents in rats. Male SD rats fed for 5 weeks on the diets containing "Mekabu", extacted sodium alginate or viscous exudate. The plasma cholesterol in sodium alginate fed rats was significanly lower than that in control rats. Dietary sodium alginate caused an enlargement of small intestine and cecum. The addition of "Mekabu", extacted sodium alginate or viscous exudate to diet increased water holding capacity of cecal contens.

“Mekabu”是在“裙带菜”（裙带菜）的根部附近形成的孢子叶。 “Mekabu”的粘性渗出物主要由海藻酸组成。本研究的目的是阐明“Mekabu”的生理作用和化学性质。从“Mekabu”中提取的粘性渗出液的粘度和海藻酸含量与“裙带菜”和“昆布”进行了比较。用E型粘度计测定粘度，用HPLC分析海藻酸含量。从“裙带菜”中提取的粘性渗出液的粘度和海藻酸含量大于从“裙带菜”或“昆布”中提取的渗出液。难消化的多糖可以在大肠中发酵产生短链脂肪酸(SCFA)。几乎所有的短链脂肪酸都被吸收并进入宿主动物的全身能量库。除了提供能量外，SCFA 对消化器官也有生理影响。因此，我们使用猪盲肠细菌在分批培养中测量了从海藻酸钠或从“Mekabu”提取的粘性渗出液中释放的气体和 SCFA 产量。培养物中微不足道的气体释放和 SCFA 产生表明海藻酸对于后肠细菌的发酵能力很差。在饮食中添加粘性膳食纤维会导致消化器官增大。膳食纤维的粘度可能在这些效应中发挥重要作用。我们观察了“Mekabu”对大鼠血浆胆固醇、消化器官和盲肠内容物的影响。雄性SD大鼠以含有“Mekabu”、提取海藻酸钠或粘性渗出液的饮食喂养5周。海藻酸钠喂养的大鼠血浆胆固醇明显低于对照大鼠。膳食海藻酸钠导致小肠和盲肠增大。在饮食中添加“Mekabu”、提取的海藻酸钠或粘性渗出液可增加盲肠内容物的持水能力。

项目成果

N.Yamanaka,N.Ogawa,T.Sakata: "Influences of Sodium Alginate Extracted from "Mekabu" (Sporopyll of Undaria pinnatifida) on Digestive Organs and Cecal Contents in Rats." Food Sci.Technol.,Int.2. 108-112 (1996)

N.Yamanaka、N.Okawa、T.Sakata：“从“Mekabu”（裙带菜孢子粉）中提取的海藻酸钠对大鼠消化器官和盲肠内容物的影响。”

N. Togari, N. Ogawa, T. Sakata.: "Poor Fermentability of "Mekabu" (Sporophyll of Undaria pinnatifida) Alginic Acid in Batch Culture Using Pig Cecal Bacteria." J. Nutr. Sci. Vitaminol.41. 179-185 (1995)

N. Togari、N. Okawa、T. Sakata.：“使用猪盲肠细菌进行分批培养时，‘Mekabu’（裙带菜孢子叶）海藻酸的发酵能力较差。”

N. Yamanaka, N. Ogawa, T. Sakata: "Influences of Sodium Alginate Extracted from "Mekabu" (Sporopyll of Undaria pinnatifida) on Digestive Organs and Cecal Contents in Rats." Food Sci. Technol., Int.2. 108-112 (1996)

N. Yamanaka、N. Okawa、T. Sakata：“从“Mekabu”（裙带菜孢子皮）中提取的海藻酸钠对大鼠消化器官和盲肠内容物的影响。”

小川宣子他: "「食品の物性第19集 卵の熱凝固性」" 食品資材研究会, 18 (1995)

小川信子等人：“食品的物理性质第 19 卷：鸡蛋的热凝固”食品材料研究组，18 (1995)

外狩なつみ,小川宣子: "「ブタ盲腸内容物を利用したバッチ培養法による食品の栄養生理学的評価」" 岐阜女子大学紀要. 24. 25-31 (1995)

Natsumi Tokari、Nobuko Okawa：“使用猪盲肠内容物进行分批培养的食品的营养和生理评价”岐阜女子大学公报 24. 25-31 (1995)。