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MOLECULAR MECHANISM OF VIRULENT FACTOR PRODUCTION IN GRAM POSITIVE BACTERIA

革兰氏阳性菌毒力因子产生的分子机制

基本信息

批准号：
08407010
负责人：
HAYASHI Hideo
金额：
$ 23.36万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1999
项目状态：
已结题

项目摘要

Molecular mechanism of production of toxins and the other virulence factors in Gram positive bacteria has been investigated to develop novel infection control measures for Gram positive bacteria which produce variety of virulence factors to cause desease in human.1. The production of toxins and enzymes was regulated by the environmental factors.The production of toxins (α, β, γ, ε, θ, etc, in Clostridium perfringens, toxin A and B in C. difficile, soluble and surface virulent factors in Staphylococcus aureus) was regulated by the growth phase, density/population of bacteria (quoram sensing), carbon source and nutritional substrate, inter-cellular factors (like pheromon). Under the stressful condition for bacteria, ie ; heat, extream pH, in the presence of heavy metals, antibiotics, the bacteria induced the stress responsible genes including virulent genes.2. Specific receptors for sensing environmental factors.Each bacterium has a certain specific receptors for environmental factors, i … More .e., the most common one is the sensor component of two-component system. In C. perfringens, several systems have been identified, among which one for activation and the other for inhibition of production of toxin. In S. aureus, the receptors for zinc, alkaline, β-lactams were mostly identified.3. Information transmitting network in bacteria.Along with genomic analysis of C. perfringens, and that of Staphylococci, molecular basis of information transmitting system in bacteria has been made clear from receptor to effecter production as an sequential "molecular net work" with global regulatory system. This information is useful for the design of antibiotics in future.4. Regulation at the level of transcription, translation, post translation and secretion from cell.On basing upon those results, molecular net work of virulent factor production has been more clearly confirmed and the results will contribute to those who are looking for more specific effective anti-infection drugs and preventive measures. Less

研究革兰氏阳性菌产生毒素和其他毒力因子的分子机制，为革兰氏阳性菌的感染控制提供新的方法.产气荚膜梭菌产生α、β、γ、ε、θ等毒素，C.艰难梭菌、金黄色葡萄球菌中的可溶性和表面毒力因子）受生长期、细菌密度/群体（quoram传感）、碳源和营养底物、细胞间因子（如信息素）调节。细菌在高温、极端pH、重金属、抗生素等胁迫条件下，诱导了包括毒力基因在内的胁迫相关基因.感受环境因子的特异性受体。每一种细菌都有一定的感受环境因子的特异性受体， ...更多信息例如，最常见的是双组分体系的传感器组分。In C.在产气荚膜杆菌中，已经鉴定了几种系统，其中一种用于激活，另一种用于抑制毒素的产生。In S.金黄色葡萄球菌主要为锌、碱性、β-内酰胺类药物的受体.沿着对C.通过对产气荚膜杆菌和葡萄球菌的研究，阐明了细菌信息传递系统从受体到效应子的分子基础是一个具有全局调控系统的有序的“分子网络”。这些信息对今后抗生素的设计具有参考价值.在转录水平、翻译水平、翻译后水平和细胞分泌水平上的调控，进一步明确了毒力因子产生的分子网络，为寻找更特异有效的抗感染药物和预防措施提供了依据。少