S19 RIBOSOMAL PROTEIN DIMER AS MONOCYTE CHEMOTACTIC FACTOR IN CHRONIC INFLAMMATION.

S19 核糖体蛋白二聚体作为慢性炎症中的单核细胞趋化因子。

• 批准号: 08457072
• 负责人: YAMAMOTO Tetsuro
• 金额: --
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457072/
• 关键词: S19 ribosomal protein; transglutaminases; chemical mediators; monocytes; macrophages; chemotactic factor; recombinant protein; rheumatoid arthritis; S19リボゾーム

The extracts of rheumatoid arthritis-synovial lesions from seven patients possessed a strong chemotactic activity for monocytes and a negligible one for polymorphonuclear leukocytes. These results are consistent with a prominent histological feature of the synovial lesion, the mononuclear cell predominant infiltration. The major monocyte chemotactic factor in the synovial tissue extracts was purified to a single protein peak in reverse phase HPLC with a C4 column. NH_2-terminal amino acid analysis of the initial twenty residues yielded a single sequence. Surprisingly, this sequence was completely identical to that of S19 ribosomal protein. The purified sample demonstrated two protein bands in SDS-PAGE with apparent molecular masses of 34 k and 68 k. These sizes were two and four times that of S19 ribosomal protein suggesting that the chemotactic factor would be a dimer or tetramer of S19 ribosomal protein cross-linked by factor XIIIa. A recombinant human S19 ribosomal protein was prepared as a fusion protein with a maltose binding protein in E.coli. After treatment with factor XIIIa, cross-linked recombinant S19 ribosomal protein exhibited the monocyte chemotactic activity although the untreated recombinant protein did not.

类风湿关节炎滑膜病变的提取物从七个病人具有很强的趋化活性的单核细胞和一个可以忽略不计的多形核白细胞。这些结果与滑膜病变的显著组织学特征，即单核细胞为主的浸润一致。滑膜组织提取物中的主要单核细胞趋化因子在具有C4柱的反相HPLC中纯化为单一蛋白峰。NH_2-末端氨基酸分析的前20个残基产生一个单一的序列。令人惊讶的是，该序列与S19核糖体蛋白的序列完全相同。纯化后的样品在SDS-PAGE中显示两条蛋白带，其表观分子量分别为34 k和68 k。这些大小是S19核糖体蛋白的2倍和4倍，这表明趋化因子将是由因子XIIIa交联的S19核糖体蛋白的二聚体或四聚体。重组人S19核糖体蛋白在大肠杆菌中被制备为与麦芽糖结合蛋白的融合蛋白。在用因子XIIIa处理后，交联的重组S19核糖体蛋白表现出单核细胞趋化活性，而未处理的重组蛋白没有。

项目成果

Hiroshi, Nishiura et al.: "Medical Aspects of Proteases Inhibitorys" ISO Press, Amsterdam(印刷中), (1997)

Hiroshi, Nishiura 等人：“蛋白酶抑制剂的医学方面”ISO Press，阿姆斯特丹（正在印刷中），（1997 年）

Hiroshi,Nishiura et al.: "Monocyte chemotactic factor in rheumatoid arthritis synovial tissue." J.Biol.Chem.271. 878-882 (1996)

Hiroshi，Nishiura 等人：“类风湿性关节炎滑膜组织中的单核细胞趋化因子。”

Hiroshi, Nishiura et al.: Medical Aspects of Proteases Inhibitors.IOS Press, Amsterdam. (in press.), (1997)

Hiroshi, Nishiura 等人：蛋白酶抑制剂的医学方面。IOS 出版社，阿姆斯特丹。

山本哲郎: "リボゾームタンパク質の架橋化による単球走化性の発現" 生化学. 69(印刷中). (1997)

Tetsuro Yamamoto：“通过核糖体蛋白交联表达单核细胞趋化性”生物化学 69（出版中）。

Hiroshi,Nishiura et al.: "Medical Aspects of Proteases Inhibitorys" IOS Press,Amsterdam(印刷中), (1997)

Hiroshi, Nishiura 等人：“蛋白酶抑制剂的医学方面”IOS Press，阿姆斯特丹（正在印刷中），（1997 年）