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Studies on The Response and Morphology of The Cortical Neurons using Double Intracellular labeling

双细胞内标记研究皮质神经元的反应和形态

基本信息

批准号：
02670048
负责人：
YAMAMOTO Tetsuro
金额：
$ 1.41万
依托单位：
Fukui Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

The present study is aimed to reveal the neuronal morphology using the retrograde or anterograde tract tracing techniques combined with intracellular staining. The experiments were performed on rats, cats and monkeys. Under pentobarbital anesthesia(30mg/kg, i. p.), several fluorescent dyes were injected into certain areas in the cerebral cortex, thalamus and deep cerebellar nuclei. After 1-2 weeks survival, animals were reanesthetized with pentobarbital(same dose)or urethane(2g/kg, i. p.)for acute experiments. Intracellular recordings were done from neurons in the cortex or the thalamus with glass microelectrodes filled with 5% HRP, 2.5% biocytin or 20% Lucifer yellow. The studies on the cat somatosensory cortex using intracellular staining could reveal the mode of the thalamic activation onto the pyramidal neurons and were published in the journal (Exp. Brain Res., 80, '90). The data from rat cortical neurons need more sample records, but preliminary reports have been appeared in the proceedings (Yamamoto et al., '90, '91), which reported the relationship between the firing properties and the morphological features of the cortical neurons. Concerning the cerebello-thalamic projection, we could report the cortical laminar distribution of the thalamic. fibers as well as firing properties of the thalamic VL neurons (Neurosci. Res., 9, '90 ; Exp. Brain Res., 87, '91). Concerning the monkey cerebello-thalamic projection, we tried double tract labeling method to demonstrate the thalamic relays of the cerebello-prefrontal responses, and we could clearly show that the mediodorsal nucleus is one of the thalamic relay nuclei of the cerebello-prefrontal projection, which will be published in the Brain Research in 1992. In the further experiments, we will try to test suitable combination of the fluorescent dyes and intracellular staining dyes.

本研究采用逆行或顺行束示踪技术结合细胞内染色，观察神经元的形态学变化。这些实验是在大鼠、猫和猴子身上进行的。戊巴比妥钠30 mg/kg麻醉下，i. p.），将几种荧光染料注射到大脑皮层、丘脑和小脑深部核的某些区域中。存活1-2周后，用戊巴比妥（相同剂量）或乌拉坦（2g/kg，i.第页）急性实验。细胞内记录是从皮质或丘脑中的神经元用玻璃微电极填充5%HRP，2.5%的biocytin或20%的荧光黄。利用细胞内染色对猫躯体感觉皮层进行的研究可以揭示丘脑激活锥体神经元的模式，并发表在期刊（Exp. Brain Res.，80、'90）。来自大鼠皮层神经元的数据需要更多的样本记录，但初步报告已出现在会议记录中（Yamamoto et al.，'90，'91），其报道了皮层神经元的放电特性与形态特征之间的关系。关于小脑-丘脑投射，我们可以报告丘脑的皮质层状分布。纤维以及丘脑VL神经元的放电特性（Neurosci.结果：9，90年;失效日期Brain Res.，87，'91）。关于猴的小脑-丘脑投射，我们尝试用双束标记法来证明小脑-前额叶反应的丘脑中继，明确地表明背内侧核是小脑-前额叶投射的丘脑中继核团之一，这一结果将于1992年发表在《Brain Research》上。在进一步的实验中，我们将尝试测试荧光染料和细胞内染色染料的合适组合。