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Role of plasma S19 ribosomal protein in thrombus resorption

血浆S19核糖体蛋白在血栓吸收中的作用

基本信息

批准号：
18590374
负责人：
YAMAMOTO Tetsuro
金额：
$ 2.57万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590374/
关键词：
S19 ribosomal protein coagulation factor XIII monocyte macrophage leukocyte chemotactic factor thrombus blood coagulation cellular fibrinolysis 単球走化因子細胞性線溶現象

项目摘要

To elucidate roles of S19 ribosomal protein present in plasma and monocytes in the thrombus resorption, we developed an experimental model in which pre-formed blood coagula was transplanted into the guinea pig peritoneal cavity. The surface of the coagula was fully covered by monocytes/macrophages within a day after the transplantation. The monocytes/macrophages on the coagula inter-connected each other forming an epithelia-like pattern. The size of coagula rapidly decreased by day 3 and disappeared by day 7.We raised anti-S19 ribosomal protein antibodies in rabbits. When the pre-formed blood coagula was centrifuged and serum was obtained, a monocyte-directed chemotactic factor was recovered in the serum. The monocyte chemotactic factor was immunologically identified to be the S19 ribosomal protein dimer. When guinea pig blood was coagulated under the presence of the anti-S19 ribosomal protein antibodies and then transplanted into the peritoneal cavity, all of the above events in the resorption process were significantly reduced. Especially, the coagula surface was covered only partly by monocytes/macrophages and the epithelia-like formation of these cells was not seen.These results strongly suggested that the S19 ribosomal protein in plasma was cross-linked by activated coagulation factor XIII resulting in the dimer formation during blood coagulation and that this dimer recruited monocytes which would involve in the coagula resorption.

为了阐明血浆和单核细胞中存在的S19核糖体蛋白在血栓吸收中的作用，我们建立了一种将预形成的血液凝固物移植到豚鼠腹膜腔内的实验模型。移植后1天内凝固区表面完全被单核/巨噬细胞覆盖。凝固层上的单核/巨噬细胞相互连接，形成上皮样结构。3天后凝固区迅速缩小，7天后消失。我们用兔抗S19核糖体蛋白抗体。当预先形成的血液凝固物被离心并获得血清时，血清中恢复了单核细胞导向的趋化因子。单核细胞趋化因子经免疫学鉴定为S19核糖体蛋白二聚体。当豚鼠血液在抗S19核糖体蛋白抗体存在下凝固，然后移植到腹膜腔内，上述吸收过程中的事件均显著减少。特别是凝固区表面仅有部分被单核/巨噬细胞覆盖，未见上皮样形成。这些结果强烈提示血浆中的S19核糖体蛋白被激活的凝血因子XIII交联，导致凝血过程中形成二聚体，该二聚体募集了参与凝血吸收的单核细胞。