Biological activity and biosynthetic mechanism of an emetic toxin produced by Bacillus cereus

蜡状芽孢杆菌催吐毒素的生物活性及生物合成机制

• 批准号: 08457086
• 负责人: OHTA Michio
• 金额: $ 4.29万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457086/
• 关键词: cereulide; Bacillus cereus; foodborne illness; toxin; ionophore; liver failure; apotosis; mitochondria; cereulide; 脂肪変性; K^+ イオノフォア

Cereulide is the causative toxin of the emetic type foodborne illness caused by Bacillus cereus. Our previous studies exhibited that it is a cyclic depsipeptide and a potassium ionophore and that it induces mitochondrial swelling in cultured HEp-2 cells. This toxin was associated with fulminant liver failure in a human case. Chemically synthesized cereulide had the same emetic effect as purified cereulide. BALB/c mice were i.p. injected with various dosed of synthetic or purified cereulide and the development of histopathological changes as well as physiological effects were examined. Hepatocytes showed the abnormality of the mitochondria such as swelling and loss of cristae. Dose-dependent increase of small fatty droplets in the degenerated hepatocytes was also observed. These microsteatotic hepatocytes were distributed in the pericentral area and spread to the intermediate and periportal areas. In the cases of lethal doses, massive degeneration of hepatocytes was prominent. These fatal lesions included massive microsteatosis and apotosis of hepatocytes. Other organs of these mice had no apparent specific pathological changes.

小脑是蜡状芽孢杆菌引起的催眠型食源性疾病的病因。我们以前的研究表明，它是循环二肽和钾离子载体，并且在培养的HEP-2细胞中诱导线粒体肿胀。这种毒素与人类病例中的暴发性肝衰竭有关。化学合成的小脑具有与纯化小脑相同的催吐作用。 BALB/C小鼠为腹膜内。检查了各种剂量的合成或纯化小脑的注射，并检查了组织病理学变化以及生理效应的发展。肝细胞表现出线粒体异常，例如肿胀和Cristae的损失。还观察到退化的肝细胞中小脂肪液滴的剂量依赖性增加。这些微蛋白酶肝细胞分布在周围的区域，并扩散到中间和周围区域。在致命剂量的情况下，肝细胞的大量变性很明显。这些致命的病变包括大量的微造成病和肝细胞的凋亡。这些小鼠的其他器官没有明显的特定病理变化。

项目成果

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