Assessment of cerebellar, vestibular and spinocerebellar effects of various factors using posuturography

使用姿势描记法评估各种因素对小脑、前庭和脊髓小脑的影响

• 批准号: 08457127
• 负责人: YOKOYAMA Kazuhito
• 金额: $ 4.74万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457127/
• 关键词: Posturography; Computer; Frequecncy analysis; Lead; Solvents; Cerebellar; Vestibular; Sarin

To examine the effects of occupational and environmental factors such as lead, organic solvents (n-hexane, xylene and toluene) and sarin on vestibular, cerebellar and spinocerebellar afferent system using a computerized posturography with sway frequency analysis. The following three groups of subjects were examined : (1) 49 male chemical factory workers exposed to lead stearate, aged 27-63 (mean 43) years, with concurrent blood lead concentrations (BPhs) of 11-113 (mean 48) mug/10Og and past mean BPbs of 7-52 (mean 24) mug/100g ; (2) 29 male sandal, shoe and leather factory workers, aged 35-73 (mean 51) years, with urinary concentrations of 2,5-hexanedione (HD) of 0.41-3.06 (mean 1.20) mg/g creatinine ; and (3) 9 males and 9 females, aged 19-58 (mean 31) years, who exposed to accidentally sarin by a presumed terrorist attack (Tokyo Subway Sarin Poisoning, March 20, 1995) and showed plasma cholinesterase (ChE) of 13-131 (mean 72) IU/1 6-8 months before the study. Control subjects for th … More e three groups were "healthy" volunteers composed of (1) 23 males, (2) 22 males, and (3) 31 males and 24 females, respectively. Postural balance was measured using a force platform connected to a microcomputer. Power of the sway for each frequency domain was calculated by the FFT method ; length and area of the sway path were measured ; and Romberg quotients (RQs, eyes-closed to eyes-open ratios) for each sway measures were determined.In lead workers, the postural sway of high (2-4 Hz) and low (0-1 Hz) frequencies with eyes open and closed were significantly increased. The sway of 0.5-2 Hz with eyes open was significantly related to concurrent BPb ; whereas the sway of high frequency with eyes closed was related to past mean BPb. In organic solvent workers, the high frequency sway with eyes closed was significantly increased ; 1-4 Hz sway with eyes open was related positively to HD and inversely to methylhippurinc acid concentrations in urine. In female sarin poisoning patients, low frequency sway with eyes open was significantly increased. RQs for low frequency sway were significantly correlated with log ChE in both male and female poisoning patients.The pattern of the posturographic changes suggested that the vestibulo- cerebellum, anterior cerebellar lobe and spinocerebellar afferent were asymptomatically affected by lead ; the vestibulo-cerebellar change reflected concurrent lead absorption and the anterior cerebellar one reflected past absorption. Similarly, the vestibulo-cerebellar and spinocerebellar afferent function was affected by n-hexane ; the effect on vestibulo-cerebellar function was possibly inhibited by xylene. The chronic (long-term) vestibulo-cerebellar effect was considered to be induced by acute sarin poisoning ; females might be more susceptible than males. The computerized posturography with frequency analysis seems useful for assessment of vestibular, cerebellar and spinocerebellar afferent function. Less

目的：应用计算机姿势图和摆动频率分析技术，研究职业和环境因素如铅、有机溶剂（正己烷、二甲苯和甲苯）和沙林对前庭、小脑和脊髓小脑传入系统的影响。本研究共检测了三组受试者：（1）49名接触硬脂酸铅的化工厂男性工人，年龄27-63岁（平均43岁），同期血铅浓度11-113 μ g/100 μ g，既往血铅浓度7-52 μ g/100 μ g，平均24 μ g/100 μ g;（2）29名男性凉鞋、皮鞋和皮革厂工人，年龄35-73岁，平均51岁，尿中2，5-己二酮（HD）浓度为0.41-3.06平均1.20 mg/g肌酐;男9例，女9例，年龄19-58岁，平均31岁，因疑似恐怖袭击意外接触沙林（东京地铁沙林中毒，1995年3月20日），并显示血浆胆碱酯酶（ChE）为13-131（平均72）IU/l 6-8个月前的研究。对照受试者 ...更多信息 三组为“健康”志愿者，分别由（1）23名男性、（2）22名男性和（3）31名男性和24名女性组成。姿势平衡的测量使用连接到一个微型计算机的力平台。通过FFT方法计算每个频域的摇摆功率;测量摇摆路径的长度和面积;确定每个摇摆测量的Romberg比值（RQ，闭眼与睁眼比率）。在铅工人中，高（2-4 Hz）和低（0-1 Hz）频率的姿势摇摆与睁眼和闭眼显著增加。睁眼时0.5 ~ 2 Hz的摆动与同期BPb显著相关，而闭眼时高频摆动与既往平均BPb相关。有机溶剂作业工人闭眼时高频摆动明显增加，1-4 Hz睁眼摆动与HD呈正相关，与尿中甲基马嘌呤酸浓度呈负相关。女性沙林中毒患者睁眼低频摆动明显增加。男女中毒者低频摆动的RQ值与ChE的log值均呈显著相关，提示铅对前庭-小脑、小脑前叶和脊髓小脑传入神经的影响是无症状的，前庭-小脑的改变反映了铅的同时吸收，小脑前叶的改变反映了铅的过去吸收。同样，前庭-小脑和脊髓小脑传入功能受到正己烷的影响，对前庭-小脑功能的影响可能被二甲苯抑制。慢性（长期）前庭-小脑效应被认为是由急性沙林中毒引起的;女性可能比男性更敏感。计算机姿势图频率分析法可用于评价前庭、小脑和脊髓小脑的传入功能。少

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