The genes of renin-angiotensin axis as a risk factor for progression of chronic glomerulonephritis

肾素-血管紧张素轴基因作为慢性肾小球肾炎进展的危险因素

• 批准号: 08670920
• 负责人: SUHARA Yutaka
• 金额: $ 1.22万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670920/
• 关键词: hematuria; chronic glomerulonephritis; angiotensin converting enzyme gene; gene polymorphism; アンギオテンシン変換酵素

Aim of the study) The relation of angiotensin converting enzyme gene polymorphism to the modes of onset of several renal glomerular diseases was studied.Methods) Genomic DNA of leukocytes was purified for PCR from 63 patients with several glomerular diseases by usual method. PCR was performed to detect insetion (I allele) or deletion (D allele) of Alu sequence in intron 16 of angiotensin converting enzyme gene. These results compared with the modes of onset in each patients.Results) In patients with IgA nephropathy, the number of DD polymorphism was dominant (II9, ID 4, DD 5, respectively), on the other hand, in patients with asymptomatic hematuria, II polymorphism was dominant ( II11, lD 10, DD 1, respectively).In patients with proteinuria at onset of glomerular diseases, DD polymorphism was dominant compared with the patients showed hematuria alone at the onset (proteinuria ; II8, ID 10, DD 6, hematuria alone ; II20, IID 16, DD 2, respectively).Conclusion) The polymorphism of angiotensin converting enzyme gene may affects the modes of onset as well as the pregression of renal glomerular diseases.

研究目的：探讨血管紧张素转换酶基因多态性与几种肾小球疾病发病方式的关系。PCR检测血管紧张素转换酶基因第16内含子Alu序列的插入（I等位基因）或缺失（D等位基因）。结果）伊加肾病患者中DD多态性的数量占优势，而DD多态性的数量占优势（II 9，ID 4，DD 5），另一方面，在无症状血尿患者中，II多态性占优势在肾小球疾病发作时有蛋白尿的患者中，与发病时仅表现血尿的患者相比，DD多态性占优势（蛋白尿：Ⅱ 8例，ID 10例，DD 6例，单纯血尿; II20，IID 16，DD 2，结论）血管紧张素转换酶基因多态性可能影响肾小球疾病的发病方式及病情进展。

项目成果

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Tsuda M. 等人：“非典型 x 连锁性肉芽肿病中嗜中性粒细胞色素 b558 中可能的线虫结合配体的新突变”人类遗传学。

栖原優,渡辺剛史,他: "生涯検尿-腎検診システムの現況と将来" 日本医学雑誌. 57・10. 455-462 (1998)

Yu Suhara、Tsuyoshi Watanabe 等：“终身尿液分析-肾脏检查系统的现状和未来”日本医学杂志 57・10（1998）。