Viral infection has involved relapse of nephrotic syndrome, onset or aggravation of chronic glomerulonephritis

病毒感染导致肾病综合征复发、慢性肾小球肾炎发作或加重

• 批准号: 10670742
• 负责人: SUZUKI Shigeo
• 金额: $ 0.83万
• 依托单位: Fukushima Medical University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670742/
• 关键词: anti-GBM nephritis; viral infection; coxsackievirus B4; IgA nephropathy; ネフローゼ症候群; コクサッキーウイルス; 肉眼的血尿; 再発; マウス実験腎炎; メサンギウム細胞; 紫斑病性腎炎

The etiology or aggravation factors of glomerulonephritis, such as IgA nephropathy, is still unclear. Episodes of nephritis are frequently proceeded by acute viral infections, therefore, we examined how viral infection would be participating in the exacerbation of anti-GBM nephritis in mice.Male BALB/c mice were injected intravenously with a single dose of anti-GBM antibody. Coxsackievirus B4 (CoxB4), in the group of enterovirus and minimum RNA virus, was inoculated intravenously into the nephritis induced mice. These mice were divided into three groups ; in group A, CoxB4 was injected intravenously on day 0, simultaneously with anti-GBM antibody injection, : in group B, CoxB4 was injected intravenously on day 7, when urinary findings worsening, : in group C (control group), anti-GBM antibody was alone injected, no challenged with virus. The amount of urine protein excretion and various cytokines were measured with time. Moreover, these mice were sacrificed, and the kidneys were collected for light microscopy, immunohistochemistory, and measurements of the message levels of various cytokines from 2 to 14 days.The amounts of urinary protein excretion were significantly increased in group A, than group B or group C.These results indicate that the course of nephritis in mice was influenced by viral infections, especially on early stage infections.

肾小球肾炎如伊加肾病的病因或加重因素尚不清楚。肾炎的发作通常由急性病毒感染引起，因此，我们研究了病毒感染如何参与小鼠抗GBM肾炎的恶化。将肠道病毒和最小RNA病毒组中的柯萨奇病毒B4（CoxB 4）静脉接种于肾炎模型小鼠。A组于第0天静脉注射CoxB 4，同时注射抗GBM抗体; B组于第7天尿液检查恶化时静脉注射CoxB 4; C组（对照组）仅注射抗GBM抗体，不进行病毒攻击。测定尿蛋白排泄量和各种细胞因子随时间的变化。结果表明，A组小鼠的尿蛋白排泄量明显高于B组和C组，说明病毒感染对小鼠肾炎的病程有影响，尤其是早期感染。