Therapeutic effect of nitric oxide on malignant glioma

作者研究了一氧化氮（NO）产生剂对培养的胶质瘤细胞生长和放射敏感性的影响。用一氧化氮（NO）产生剂S-亚硝基-N-乙酰青霉胺（SNAP）和硝普钠（SNP）处理三种胶质瘤细胞系C6、T98 G和U87。它们引起亚硝酸盐在细胞培养基中的积累，并抑制胶质瘤细胞的生长。这种生长抑制作用减弱血红蛋白，一种已知的抑制剂NO，表明生长抑制作用是由NO介导的。当C6细胞照射在SNAP或SNP在160 μ M的存在下，观察到放射增敏。在10%存活率时，敏化剂增强比分别为3和2.5。因此，NO生成剂可能是一种潜在的恶性胶质瘤细胞生长抑制剂和放射增敏剂。NO的调节可能为恶性胶质瘤的治疗提供新的途径。

The authors examined the effect of nitric oxide (NO) generating agents on the growth and radiosensitivity of the cultured glioma cells. Three glioma cell lines, C6, T98G and U87 were treated with the NO generating agents, S-nitroso-N-acetyl-penicillamine (SNAP) and sodium nitroprusside (SNP). They caused nitrite accumulation in the cell culture media and inhibited the growth of the glioma cells. This growth-inhibitory effect was attenuated by hemoglobin, a known inhibitor of NO,suggesting that the growth inhibitory effect was mediated by NO.When C6 cells were irradiated in the presence of SNAP or SNP at 160 muM,radiosensitization was observed. The sensitizer enhancement ratio at 10% survival was 3 and 2.5, respectively. We conclude that NO generating agent can be a potential growth inhibitor and radiosensitizer for the malignant glioma cells. NO modulation might provide a novel therapeutic approach for malignant glioma.