THE EFFECT OF VOLATILE ANESTHETICS ON ENDOTHELIAL VASODILATION PROPERTY

挥发性麻醉药对内皮血管舒张性能的影响

• 批准号: 08671764
• 负责人: IRANAMI Hiroshi
• 金额: $ 1.47万
• 依托单位: WAKAYAMA MEDICAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671764/
• 关键词: nitric oxide; volatile anesthetics; vascular dilation; phospholipase; G protein; 揮発性麻酔薬; 血管弛緩反応

The current study was aimed to clarify the signal transduction mediating the phospholipase A2- stimulated EDRF production which is one of the major mechanism underlying endothelial vascular tone control via basally released EDRF and to examine the effects of volatile anesthetics on the vascular dilation phenomen.The results of the current study indicated that (1) activated phospholipase A2 directly by melittin or indirectly by A1F and vanadate mediated EDRF release from endothelium by means of endothelial Ca2+/Calmoduline-independent nitric oxide synthase, in which arachidonate metabolites by lypoxygenase and cytochrome P450 played possible crucial roles, (2) halothane but not isotlurane or sevoflurane inhibited this relaxation mechanism stimulated by direct activation of phospholipase A2 with mellitin and (3) no anesthetics inhibited this relaxation mechanism stimulated by indirect activation of phospholipase A2 with G protein activators, A1F and vanadate.Accordingly, the current study showed the novel signal prunsduction mediating endothelial phospholipase A2-induced EDRF production and inhibitory action of halothane, unlike other anesthetics, on this mechanism . The findings in the current study will be substituted to the pharmacological papers.

本研究的目的是阐明磷脂酶A2刺激EDRF产生的信号转导途径，并观察吸入麻醉药对血管舒张的影响。蜂毒肽直接激活磷脂酶A2或A1 F间接激活磷脂酶A2，钒酸盐通过内皮钙/钙调素非依赖性一氧化氮合酶介导内皮释放EDRF，其中脂氧合酶和细胞色素P450对花生四烯酸代谢可能起关键作用，（2）氟烷而不是异氟烷或七氟烷抑制了由蜂毒肽直接激活磷脂酶A2刺激的这种舒张机制，（3）没有麻醉药能抑制这种由G蛋白激活剂A1 F和钒酸盐间接激活磷脂酶A2所引起的舒张机制，因此，本研究发现了一种新的信号转导途径介导内皮磷脂酶A2诱导EDRF的产生，氟烷对这种机制的抑制作用不同于其他麻醉药。当前研究的结果将被药理学论文取代。

项目成果

Hiroshi Iranami: "A beta-adrenoceptor agonist evokes a nitric oxide-cGMP relaxation mechanism modulated by adenylyl cuclase in rat aorta" ANESTHESIOLOGY. 85. 1129-1138 (1996)

Hiroshi Iranami：“β-肾上腺素受体激动剂在大鼠主动脉中激发由腺苷酸环化酶调节的一氧化氮-cGMP 松弛机制”麻醉学。

Hiroshi Iranami: "Possible contribution of transmembrane Ca2+ to adenylate cyclase-mediated NO-cGMP relaxation in rat aorta" ANESTHESIOLOGY. 87. 712-713 (1997)

Hiroshi Iranami：“跨膜 Ca2 对腺苷酸环化酶介导的大鼠主动脉 NO-cGMP 松弛的可能贡献”麻醉学。

Hiroshi Iranami: "Halothane inhibition of acetylcholine-induced relaxation in rat mesenteric artery and aorta" Can J Anesth. 44. 1196-1203 (1997)

Hiroshi Iranami：“氟烷抑制乙酰胆碱诱导的大鼠肠系膜动脉和主动脉松弛”Can J Anesth。

Hiroshi Iranami: "Halothane inhibition of acetylcholine-induced relaxation in rat mesenteric artery and aorta" Can J Anaesth. 44. 1196-1203 (1997)

Hiroshi Iranami：“氟烷抑制乙酰胆碱诱导的大鼠肠系膜动脉和主动脉松弛”Can J Anaesth。

Hiroshi Iranami: "Possible contribution of transmembrane Ca2+ influx to adenylate cyclase-mediated NO-cGMP relaxation in rat aorta" ANESTHESIOLOGY. 87. 712-713 (1997)

Hiroshi Iranami：“跨膜 Ca2 流入对大鼠主动脉腺苷酸环化酶介导的 NO-cGMP 松弛的可能贡献”麻醉学。