Mechanisms of oxidative stress in the human arteries from patients with metabolic syndrome and the treatment using a gene therapy

我们用2. 5 mm长的人网膜动脉，无内皮。暴露于高浓度的D-葡萄糖（22. 5 mmol/L）可降低ATP敏感性K+通道开放剂引起的舒张和超极化反应。在高糖环境下，人血管平滑肌细胞内超氧化物水平增加，细胞膜上NADPH氧化酶亚基p47 phox表达增加，同时伴随着平滑肌细胞内F-actin比值的增加

We prepared arterial rings with 2. 5 mm length of human omental arteries without endothelium. Exposure toward a high concentration of D-glucose(22. 5 mmol/L) reduced both relaxation and hyperpolarization in response to an ATP-sensitive K+channel opener. In human vascular smooth muscle cells exposed to high glucose, intracellular levels of superoxide in addition to expression of NADPH oxidase subunit p47phox on cellular membrane increased and they were accompanied by the enhanced F-actin ratio in the smooth muscle cells