The meaning of the measurement of oligosaccharide antigens and its regulatory factors as a tumor marker for malignant progression of bladder cancer

寡糖抗原及其调控因子测定作为膀胱癌恶性进展肿瘤标志物的意义

• 批准号: 08671839
• 负责人: YAMADA Takumi
• 金额: $ 1.22万
• 依托单位: Saitama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671839/
• 关键词: sialic acid; sialyl Lewis X; fucosiltransferase; bladder cancer; 膀胱腫瘍; フラシルトランスフェラーゼ; 悪性進展; シアリルLewis X

Objective : The aim of study is to investigate the relation of expression of sialic acid and sialyl Lewis x in bladder cancer cells to malignant progression of the bladder cancer and to indicate simple and useful tumor markers. Results : 1. Sialic acid levels of the tissue of bladder tumors (663.2*399.4 mg/mg.protein) were higher than those of renal cancer, normal kidney and normal urothelium (110.4, 181.5 and 73.3 mg/mg.protein respectively). Sialyl Lewis X values of the tissue of high grade bladder tumors (1039 mg/mg.protein and 1468 mg/mg.protein) were higher than those of renal cancer, normal kidney and low grade bladder tumor (653, 664 and 507 mg/mg.protein respectively). 2. The mean levels and a median value of sialic acid in urine of the patients with bladder tumor was 10.6*3.4 mg/dl and 10 mg/dl respectively. Those were significantly higher than the respective values of sialic acid in urine of the subjects with microscopic protein derived from various urological diseases, excluding bladder cancer. When the cut-off level being set at 8 mg/dl, sialic acid in urine had a sensitivity of 100%, a specificity of 81.8%, a positive predictive value of 67.7% and a negative predictive value of 100%. 3.Immunohistological study with anti-Lewis X antibody revealed positive staining in 6 of 7 specimens of bladder tumor. Although strong amplification of Fuc-T III, V, VI and VII were detected by RT-PCR in all the bladder tumors, these results did not correspond to histological grade and sialyl Lewis X expression of the tumors.

目的：探讨唾液酸和唾液酸化Lewis x在膀胱癌细胞中的表达与膀胱癌恶性进展的关系，寻找简便、实用的肿瘤标志物。结果：1.膀胱癌组织唾液酸水平(663.2±399.4 mg/mg蛋白)高于肾癌、正常肾和正常尿路上皮组织(分别为110.4 mg/mg蛋白、181.5 mg/mg蛋白和73.30 mg/mg蛋白)。高级别膀胱癌组织唾液酸化Lewis X值(1039 mg/mg蛋白和1468 mg/mg蛋白)高于肾癌、正常肾和低级别膀胱肿瘤组织(分别为653 mg/mg蛋白、664 mg/mg蛋白和507 mg/mg蛋白)。2.膀胱癌患者尿液唾液酸平均水平为10.6±3.4 mg/dl，中位数为10 mg/dl。这些都明显高于尿液中唾液酸值，这些微量蛋白来自各种泌尿系统疾病，不包括膀胱癌。以8 mg/dl为临界值，尿液唾液酸检测的敏感性为100%，特异性为81.8%，阳性预测值为67.7%，阴性预测值为100%。3.用抗Lewis X抗体进行免疫组织化学检测，7例膀胱癌中6例阳性。尽管RT-PCR检测到Fuc-TⅢ、V、VI和VII在所有膀胱肿瘤中均有强扩增，但这些结果与肿瘤的组织学分级和唾液酸化Lewis X的表达不一致。