Molecular biological study to evaluate the function of growth factors and treament in ocular angiogenesis

研究了激光诱导大鼠脉络膜新生血管形成（CNV）过程中生长因子mRNA的表达。在新生血管病变细胞中，观察到碱性成纤维细胞生长因子（bFGF）及其受体转化生长因子（TGF - β 2） mRNA及其受体的表达。我们的研究结果表明，内源性bFGF和tgf - β通过自分泌和旁分泌机制促进CNV。在CNV中观察到VEGF的免疫反应性。RT-PCR和Western blotting证实了这一结果。我们发现巨噬细胞是VEGF的主要来源。我们成功地治疗实验性脉络膜新生血管给予TNP，干扰素- β， tecogalan。我们证明了HVJ脂质体方法可以有效地将基因转移到大鼠脉络膜新生血管中。VEGF反义寡核苷酸在脉络膜新生血管病变中特异性转移到细胞中。HVJ脂质体法为CNV的基因治疗提供了一种高效的手段。我们将bFGF基因或bFGF反义基因诱导入培养细胞（RPE）。这些细胞系可用于移植治疗CNV。在大鼠视网膜静脉闭塞模型缺血视网膜中表达bFGF及其受体mrna，在眼压引起的短暂性视网膜缺血中表达bFGF及其受体mrna。这些结果表明，缺血调节这些因素。

We investigated mRNA expression of growth factors in laser induced experimental choroidal neovascularizon (CNV) in rats. Expression of basic fibroblast growth factor (bFGF) and its receptor, transforming growth factor (TGF) -beta (mainly TGF-beta2) mRNA and its reseptor was observed markedly in cells of neovascular lesions. Our findings indicated that endogenous bFGF and TGF-beta promotes CNV through autocrine and paracrine mechanism. Immunoreactivity for VEGF was markedly observed in CNV.This results was confirmed by RT-PCR and Western blotting. We revealed that macrophages is the mainsource of VEGF.We successfully treated experimenral choroidal neovascularization with administration of TNP,interferon-beta, tecogalan.We demonstrated that HVJ liposome method can transfer genes efficiently into choroidal neovascularization in a rat model. VEGF antisense oligonucleotides were specifically transferred into cells in the lesions of choroidal neovascularization. HVJ liposome method provides a highly efficient means of gene therapy for CNV.We induced bFGF gene or bFGF antisense gene into cultured (RPE). These cell lines would be useful for the transplantation to treat CNV.bFGF and its receptor mRNAs were expressed in the ischemic retina in a rat model of retinal vein occlusion, and bFGF and its receptor, midkine mRNAs were expressed in transient retinal ischemia induced by ocular pressure. These results suggest that ischemia regulates these factors.