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Using senescence-accelerated mouse (SAM) strains and aged C57/BL/6 mice, we examined the senescence-related changes of mucosal immune responses.

使用衰老加速小鼠 (SAM) 品系和老年 C57/BL/6 小鼠，我们检查了粘膜免疫反应的衰老相关变化。

基本信息

批准号：
14571744
负责人：
SASAKI Minoru
金额：
$ 1.86万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

The obtained findings are as follows:1. Mitogenic responses of the splenic B cells from senescence-prone (SAM-P) mice were significantly lower than those of senescence-resistant (SAM-R) mice, although the kinetics was not altered2. The polyclonal B cell activation induced by Porp/iyromonas gingivalis LPS in SAM-P mice was also diminished. However, the alteration in SAM-P mice occurred rehitively earlier than the reduction of mitogenic responses in SAM-P mice.3. Analysis of ovalbumin (OVA)-specific antibody-forming B cell responses in aged (12-month-old and 24-month-old) mice immunized orally with OVA and mucosal adjuvant (cholera toxin) revealed the significant reductions in mucosal immune responses (antigen-specific IgA antibody-forming B cell responses) in Peyer's patches and in lamina propria, as well as systemic immune responses in spleen.4. However, development of the senescence-related alterations could occur earlier in mucosal immune system than in the systemic immune compartment.5. Flow cytometric analysis of T cell phenotypes indicated that the percentage of the T cell subset with extrathymic properties in SAM-P mice was significantly higher than that in SAM-R mice.Taken together, these findings indicated that both the reduction of B cell responses and the alteration in T cell subsets could occur in aged mice, which may reflect a senescence-related down-regulation in mucosal immune responses in aged mice.

所得结果如下：1。衰老易感小鼠（SAM-P）脾B细胞的有丝分裂反应明显低于抗衰老小鼠（SAM-R），尽管动力学没有改变2。LPS对SAM-P小鼠多克隆B细胞的激活作用也明显减弱。然而，这种改变在SAM-P小鼠中发生的时间明显早于SAM-P小鼠有丝分裂反应的减少。对老龄小鼠（12月龄和24月龄）口服OVA和黏膜佐剂（霍乱毒素）免疫的卵清蛋白（OVA）特异性抗体形成B细胞反应的分析显示，Peyer's斑块和固有层的粘膜免疫反应（抗原特异性IgA抗体形成B细胞反应）以及脾脏的全身免疫反应显著降低。然而，与衰老相关的改变在粘膜免疫系统中可能比在全身免疫室中更早发生。流式细胞术分析T细胞表型表明，SAM-P小鼠中具有胸腺外特性的T细胞亚群百分比显著高于SAM-R小鼠。综上所述，这些发现表明，老年小鼠的B细胞应答降低和T细胞亚群的改变都可能发生，这可能反映了老年小鼠粘膜免疫应答的衰老相关下调。