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Molecular mechanism of synatic vesicle exocytosis-Role of vesicle transport in synaptic plasticity

突触小泡胞吐作用的分子机制-小泡运输在突触可塑性中的作用

基本信息

批准号：
10215204
负责人：
SASAKI Takuya
金额：
$ 22.08万
依托单位：
The University of Tokushima Graduate School of Medicine (2000-2001)Osaka University (1998-1999)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

项目摘要

Long-term potentiation (LTP) is believed to provide an important key to understanding the cellular and molecular mechanisms by which memories are formed and stored. Numerous studies strongly suggest that important mechanisms underlying LTP involves alternations in the release of glutamate from the presynaptic cells and the resposiveness of the postsynaptic glutamate receptors. Neurotransmitters including glutamate are released by synaptic vesicle exocytosis which is regulated by many components They are classified into essential and modulatory ones for neurotransmitter release. Rab3A small G protein and Doc2 have been shown to be modulatory components which regulate Ca^<2+>-dependent synaptic vesicle exocytosis. Rab3A cycles between the GDP-bound inactive form and the GTP-bound active form and translocates between the cytosol of the presynaptic nerve terminal and the membranes of synaptic vesicles and the presynaptic plasma membrane. The activation and the translocation are regulated b … More y three regulators (Rab GDIα, Rab3 GAP, Rab3 GEP). Our studies on Rab GDIα-deficient mice indicate that this protein is not essential for basal neurotransmitter release but regulates synaptic plasticity. In contrast, Rab3 GEP-deficient mice show much more severe phenotypes and die immediately after birth due to respiratory insufficiency. We isolated a novel protein that was co-immunoprecipitated with Rab3 GEP and GAP by their respective antibodies from the crude synaptic vesicle fraction of rat brain. The protein, named rabconnectin-3, bound at least both Rab3 GEP and GAP. Doc2 interacts with Munc13 in a diacylglycerol-dependent manner, and this interaction is involved in the docking process. Our studies on Doc2-deficient mice indicate that this protein is not essential for basal neurotransmitter release but regulates synaptic plasticity Further studies on the Rab3A and Doc2 systems are necessary for understanding of the molecular mechanism of synaptic plasticity, which may underlie learning and memory Less

长时程增强(LTP)被认为是理解记忆形成和存储的细胞和分子机制的重要关键。大量研究表明，LTP的重要机制涉及突触前细胞谷氨酸释放的变化和突触后谷氨酸受体的反应性。神经递质包括谷氨酸，是通过突触囊泡的胞吐作用释放的，受多种成分的调节，分为必需的和调节性的两类。Rab3A小G蛋白和Doc2是调节依赖于Ca~(2+)的突触囊泡胞吐的调节成分。Rab3A在GDP结合的非活动型和GTP结合的活动型之间循环，并在突触前神经末梢的胞浆、突触小囊膜和突触前质膜之间移位。激活和转位受…调控三个以上的调节器(RAB GDIα、Rab3 GAP、Rab3 GEP)。我们对RABGDIα缺陷小鼠的研究表明，该蛋白对基础神经递质的释放不是必需的，但调节突触的可塑性。相比之下，Rab3GEP缺陷小鼠表现出更严重的表型，并在出生后立即死于呼吸功能不全。我们从大鼠脑突触泡粗提物中分离到一个与Rab3、GEP和GAP抗体免疫共沉淀的新蛋白。该蛋白命名为RabConnectin-3，至少与Rab3 GEP和GAP结合。Doc2与Munc13以二酰甘油依赖的方式相互作用，这种相互作用参与了对接过程。我们对Doc2基因缺陷小鼠的研究表明，该蛋白不是基础神经递质释放所必需的，而是调节突触可塑性的。进一步研究Rab3A和Doc2系统对于理解突触可塑性的分子机制是必要的，这可能是学习和记忆不足的基础