Molecular basis of water soaking of strawberry

草莓水浸的分子基础

• 批准号: 528606164
• 负责人: Professor Dr. Henryk Flachowsky
• 金额: --
• 依托单位: Institut für Gartenbauliche Produktionssysteme
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/528606164?language=en
• 关键词: Molecular; basis; water; soaking; strawberry

Strawberry is a highly perishable fruit crop species of global importance. The quality of field-grown strawberries is often severely compromised by rain. An important disorder arising from rain is ‘water soaking’. The symptomatic fruit surface has a light greyish color and a translucent and deliquescent appearance. Water soaking is associated with microcracking of the cuticle and anthocyanin leakage from the epidermal cells. The incidence of fruit rots pre- and postharvest is also increased causing significant economic loss. As a result, strawberry production is shifting from open-field, to semi- or fully-protected in plastic tunnels or greenhouses. Genotypes differ in susceptibility to water soaking. The physiological, genetic and molecular bases for such differences are not understood. The objective of the project is to determine the bases of the differential susceptibility of genotypes. (1) We will establish a developmental time course of differential gene expression (RNA-seq), water soaking, cuticle deposition, strain development and microcracking for a pair of parental genotypes selected for their contrasting susceptibilities to water soaking. (2) The parental genotypes will be crossed to produce an F1-population (200 individuals) to perform (i) phenotyping of the segregating F1-population for susceptibility to water soaking, (ii) genotyping for developing genetic linkage maps, (iii) QTL analyses and (iv) develop PCR-based molecular markers flanking the QTL regions for marker-assisted breeding. (3) We will select the most promising candidate genes (CGs) and compare the CGs identified by differential gene expression with those located in the QTL regions. This will allow establishment of a first molecular model for tolerance to water soaking. The model including the identified CGs will be verified by further expression studies using qRT-PCR in independent growing seasons. Finally, we will sequence the most promising CGs in parental genotypes, identify structural variations and develop PCR-based markers for the detection of selected structural variations. The structural variations and their association with water soaking will be verified using the F1-population. These results will help identify the genetic and molecular bases of susceptibility to water soaking in strawberry. Furthermore, provided that significant QTLs are found, new strawberry cultivars that are less susceptible to water soaking may be developed. In addition, these cultivars are likely to be less prone to fruit rots. As a consequence, the sustainability of strawberry production in the open field will be improved.

草莓是一种极易腐烂的全球重要果树品种。大田种植的草莓的质量经常受到雨水的严重影响。雨引起的一种重要的紊乱是“浸水”。有症状的水果表面呈淡灰色，外观半透明和潮解。浸水与角质层微破裂和表皮细胞花青素渗漏有关。果实采前和采后腐烂的发生率也有所增加，造成重大的经济损失。因此，草莓生产正从露地转向半保护性或完全保护性的塑料隧道或温室。不同的基因型对水浸泡的敏感性不同。这种差异的生理、遗传和分子基础尚不清楚。该项目的目标是确定不同基因型的差异易感性的基础。(1)我们将为两个对水浸泡敏感性不同的亲本建立差异基因表达(RNA-seq)、水浸泡、角质层沉积、菌株发育和微裂纹的发育时间进程。(2)杂交产生一个F1群体(200个个体)，用于(1)对分离的F1群体进行浸水感受性表型鉴定，(2)建立遗传连锁图谱，(3)QTL分析，(4)在QTL区域两侧开发基于PCR的分子标记，用于分子标记辅助育种。(3)筛选出最有希望的候选基因，并将差异表达的候选基因与位于QTL区域的候选基因进行比较。这将有助于建立第一个耐水浸泡的分子模型。包括已识别的CGs的模型将通过在独立生长季节使用qRT-PCR进行进一步的表达研究来验证。最后，我们将对亲本中最有希望的CG进行测序，识别结构变异，并开发基于PCR的标记，用于检测选定的结构变异。结构变化及其与水浸泡的关联将使用F1群体进行验证。这些结果将有助于确定草莓对水浸敏感的遗传和分子基础。此外，如果发现了显著的QTL，就有可能培育出对水浸不太敏感的草莓新品种。此外，这些品种很可能不太容易发生果实腐烂。因此，露地草莓生产的可持续性将得到提高。