Research Resource for Integrated Glycotechnology

复合碳水化合物修饰细胞表面，形成细胞外基质， 修饰分泌的糖蛋白。在这里，它们介导了各种各样的过程，这些过程对正常的 生命过程的执行。它们还进入异常过程，如病原体的入侵和 正常细胞的致癌性转化。了解碳水化合物结构的多样性， 其起源的基础和其识别的分子基础对于诊断和 疾病的治疗。在本提案中，我们概述了一个综合糖技术的研究资源， 通过开发新的方法来研究碳水化合物-蛋白质相互作用， 分子水平，并通过合作，服务和培训活动传播这些方法。 在分子水平上表征碳水化合物-蛋白质相互作用不是一件容易的事情。 碳水化合物的结构本身是非常多样的。此外，了解它们的相互作用，并使用 了解干预疾病过程，不仅需要碳水化合物的信息， 决定它们合成和识别的蛋白质。解决这种规模的特征化问题 和复杂性需要一套基于广泛的分析、生物化学、化学和 计算科学这些工具在集成以生成高效且多功能的 资源，可以回答新的碳水化合物-蛋白质相互作用系统的发现所产生的问题。 拟议的努力包括开发生产足够数量蛋白质的技术 允许物理和生物化学表征，引入碳水化合物合成方法 具有足够的多样性和纯度来探测亲和性的分子基础，开发新的工具， 糖-蛋白质相互作用的质谱和核磁表征 共振，并为数据集成到一个完整的计算平台的发展 蛋白质-碳水化合物相互作用的分子图像。

Complex carbohydrates elaborate the surfaces of cells, contribute to the extracellular matrix, and decorate excreted glycoproteins. Here they mediate a wide variety of processes that are essential to the normal execution of life processes. They also enter into abnormal processes, such as the invasion of pathogens and the oncogenic transformation of normal cells. Understanding the diversity of carbohydrate structure, the molecular basis for its origin, and the molecular basis for its recognition are extremely important to the diagnosis and treatment of disease. In this proposal we outline a Research Resource for Integrated Glycotechnology that will serve the community through the development of new approaches to study carbohydrate-protein interactions at a molecular level and the dissemination of these approaches through collaborative, service, and training activities. Characterization of carbohydrate-protein interactions at a molecular level is not an easy task. Carbohydrate structures are in themselves very diverse. Moreover, understanding their interactions, and using that understanding to intervene in disease processes, requires information on not only the carbohydrates, but also the proteins that dictate their synthesis and recognition. Tackling a characterization problem of this magnitude and complexity requires a diverse set of tools based on a wide range of analytical, biochemical, chemical, and computational science. These tools are most useful when integrated to produce an efficient and versatile resource that can answer questions that arise with the discovery of new carbohydrate-protein interacting systems. Proposed efforts include development of techniques for the production of proteins in adequate amounts to allow physical and biochemical characterization, introduction of methods for the synthesis of carbohydrates with sufficient diversity and purity to probe the molecular basis of affinity, the development of new tools for the structural characterization of the carbohydrate-protein interactions by mass spectrometry and nuclear magnetic resonance, and the development of a computational platform for the integration of data into a complete molecular picture of protein-carbohydrate interactions.