Molecular and Cellular Biology in Mechanism of Body Fluid Homeostasis (Analysis of Inter- and Intra-Cellular Signalling in the Neurosecretory Cells)

The paraventricular Nucleus （PVN） and the supraoptic Nucleus （SON） in the hypothalamus are known to be one of integrative sites that regulate immune， endocrine and autonomic nervous system。在PVN和Son秘密荷尔蒙进入系统循环中的大分子神经细胞。The Parvocellular神经细胞在PVN秘密荷尔蒙进入门户容器中，也直接影响大脑和脊柱中的自主前神经细胞。“PVN和SON合成精氨酸血管紧张素和氧化物中的大分子神经恢复细胞”。它们的发射速率与神经催眠症中的荷尔蒙数量密切相关，它们是从神经催眠症中释放的。我们研究了PACAP、前列腺素（PGs）、硝酸氧化物（NO）、ATP、腺苷、谷氨酸、伽马氨酰胺酸（GABA）、丙醇（anesthetic Drug）对离子通道和神经活动的影响，如PACAP、前列腺素（PGs）、硝酸盐（NO）、ATP、腺苷、谷氨酸和全细胞贴片-钳记录。我们的数据显示了各种生物物质，如异常和抑制性氨基酸（谷氨酸、GABA）、肽、NO、PGs和纯净物质，可以调节神经细胞中的离子电流和神经活性。我们还研究了神经细胞中的inte-and intracellular signal transduction，使用intracellular Ca-y D12+ intracellular D1成像技术和生物活性物质基因和受体的各种表达方式。These results provide new insight into understanding of the characteristics of the neurosecretory cells and the physiological implication of bioactive substances in the neuroroendocrine system.（神经内分泌系统中的生物活性物质）

The paraventricular nucleus (PVN) and the supraoptic nucleus (SON) in the hypothalamus are known to be one of integrative sites that regulate immune, endocrine and autonomic nervous system. The magnocellular neurosecretory cells in the PVN and the SON secrete hormones into the systemic circulation. The parvocellular neurosecretory cells in the PVN secrete hormones into the portal vessels in the median eminence and also directly influence autonomic preganglionic cells in the brainstem and spinal cord. The magnocellular neurosecretory cells in the PVN and the SON synthesize arginine vasopressin and oxytocin. Their firing rate is closely related to the amount of hormones released from the axon terminals in the neurohypophysis. we examined the effects of biological substances such as PACAP, prostaglandins (PGs), nitric oxide (NO), ATP, adenosine, glutamate, gamman aminobutyric acid (GABA), propofol (anesthetic drug) on the ion channels and neuronal activities in the rat slice preparation or acute dissociated neurosecretory cells, using extracellular, intracellular and whole cell patch-clamp recordings. Our data showed that various biological substances such as excitatory and inhibitory amino acids (glutamate, GABA), peptides, NO, PGs and purinergic substances modulate the ionic currents and neuronal activities in the neurosecretory cells. We also examined the inte- and intracellular signal transduction in the neurosecretory cells, using intracellular CaィイD12+ィエD1 imaging technique and the expression of various kinds of bioactive substance genes and receptors. These results provide new insight into understanding of the characteristics of the neurosecretory cells and the physiological implication of bioactive substances in the neuroendocrine system.