Thermodynamic Analysis of Protein-Ligand interactions

许多蛋白质的主要生物学功能是结合小分子。例如，酶结合底物和效应分子，而转运蛋白如血红蛋白或储存蛋白如肌红蛋白结合氧。几乎所有的生物功能都涉及这些小分子的相互作用，这些小分子作为代谢物，调节剂和信号与进行细胞过程的蛋白质的特定表面。因此，了解这种相互作用的机制对于在分子水平上理解生物过程至关重要。在大多数情况下，这种结合涉及在称为配体的小分子和称为结合位点的蛋白质表面上或附近的某些特定区域之间形成某种非共价键。蛋白质-配体复合物与它们的解离组分处于热力学平衡，在许多情况下，可以测量这些反应的热力学参数。 关于我们 基本的热力学关系表明，对感兴趣的分子的体积研究可以产生有用的新信息。蛋白质-配体复合物的固有体积Vm（周围溶剂分子不可接近的溶质的几何体积）和固有压缩性Km（溶剂不可接近的蛋白质核心的压缩性）反映了组分的固有堆积，并且由溶剂不可接近的蛋白质内部的分子间相互作用确定。因此，在蛋白质结构的配体结合诱导的任何变化应反映在Vm和Km的值。在本研究项目中，蛋白质-配体复合物的性质作为溶液条件的函数，包括溶剂化的作用，其特征在于体积的研究。直到最近，由于缺乏具有必要灵敏度的现成仪器，对生物学上感兴趣的分子的此类研究一直受到限制。我们改进了仪器，并建立了一个高灵敏度，小体积密度，声学和高压光谱仪器，使生物分子进行了广泛的体积研究。使用这些仪器，我们已经获得了独特的见解的分子间识别事件，调节生物分子过程的分子起源。少

A major biological function of many proteins is the binding of small molecules. For examples, enzymes bind substrates and effector molecules, and transport proteins such as hemoglobin or storage proteins such as myoglobin bind oxygen. Almost all biological functions involve the interactions of those small molecules that serve as metabolites, regulators, and signals with the specific surfaces of the proteins that carry out cellular processes. For this reason, an understanding of the mechanisms of such interactions is essential to a comprehension of biological process at the molecular level. In most cases, such binding involves the formation of some kind of noncovalent bond between the small molecules called as ligand and some specific region on or near the surface of the protein called as the binding site. The protein-lgand complexes are in thermodynamic equilibrium with their dissociated components, and in many cases it is possible to measure the thermodynamic parameters for these reac … More tions.Fundamental thermodynamic relationships reveal that volumetric studies on molecules of interest can yield useful new information. The intrinsic volume, Vm, (the geometric volume of a solute not accessible to surrounding solvent molecules) and the intrinsic compressibility, Km, (compressibility of the solvent-inaccessible protein core) of a protein-ligand complex reflect the intrinsic packing of the constituent and are detemined by intermolecular interactions within the solvent-inaccessible protein interior. Consequently, any changes in protein structure induced by ligand binding should be reflected in the values of Vm and Km.In this research project, the properties of protein-ligand complex as a function of solution conditions, including the role of solvation have been characterized by volumetric studies. Until recently, such studies on biologically interesting molecules have been limited because of the lack of readily available instrumentation with the requisite sensitivity. We have improved the instruments and constructed a highly sensitive, small-volume densimetric, acoustic and high-pressure spectroscopic instrumentation which enabled biological molecules to be subjected to a wide range of volumetric studies. Using these instruments, we have obtained unique insights into the molecular origins of the intermolecular recognition events that modulate biomolecular processes. Less