Analysis of the function of bone marrow stromal cell antigen, BST-1 (CD157) to support B cells

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(1) Roles for BST-1 in arthritis induced by type 2 collagen : Soluble BST-1 transgenic mice (a line S15) and wild type mice (n=6 and 8, respectively), which had been backcrossed with DBA/1J for 8 generations, were immunized with 100μg of bovine type 2 collagen and incomplete Freund adjuvant. Although the incidence of arthritis in S15 mice increased from those of wild mice. The incidence of arthritis in BST-1KO mice, which had been backcrossed with DBA/1J (N=15) for 5generations ,was higher than that of wild mice (n=13) at day 57 (100% and 69%, respectively). There was no difference in the severity. BST-1 does not have important roles in collagen induced arthritis.(2) Generation of BST-1/CD38 double knockout mouse : ES cell clones deleted both CD38 and BST-1 genes were selected and the chimeric mice were obtained. Analysis of F1 generation revealed successful targeting of CD38 and BST-1 genes will be obtained by crossing the mice of F1 generation.(3) Receptor function :Crosslinking of BST-1 on the surface of mBST-1BAF, a pro B cell line transfected with murine BST-1 cDNA, resulted in the cell aggregation, indicating that signals through BST-1 induce cell adhesion.(4) BST-1Ligand: cDNA library for expression cloning was prepared form a B cell line which binds BST-1. Candidates of cDNA for human BST-1ligand has not been obtained after screening of 2x10ィイD14ィエD1 clones.