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Study on the central nervous system of rat with systemic inflammatory response syndrome (SIRS)

全身炎症反应综合征（SIRS）大鼠中枢神经系统的研究

基本信息

批准号：
10671177
负责人：
HASE Takanori
金额：
$ 1.09万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671177/
关键词：
systemic inflammatory response syndrome c-Fos c-Jun phosphorylated c-Jun immunohistochemistry rat brain c-fos、敗血症性脳症 CNS SIRS Rat CLP

项目摘要

[BACKGROUND](1) In recent year, sepsis has accepted as a systemic inflammatory response syndrome (SIRS) that manifested changes in the body temperature, heart rate, respiratory rate and leukocyte count. These manifestations show clearly that humoral and neuronal regulation is deeply involved in the host response in this situation. Therefore, insights into changes in the central nervous system (CNS) are of value to further our understanding of pathophysiology involved in septic states. (2) C-Fos and c-Jun are inducible transcription factors (ITFs) encoded by c-fos and c-jun, respectively. The transcription activity of c-Jun are widely used as metabolic markers of activated neurons.[PURPOSE]A study was conducted determine how SIRS states affect CNS, using an antibody against c-Fos, c-Jun and phosphorylated c-Jun.[RESULTS and DISCUSSION]The immunohistochemical examination of rat brain at 8 h after SMAO demonstrated c-Fos, c-Jun and p-c-Jun IR in the specific nuclei including the central and basolateral amygdaloid nu. of the limbic system, the paraventricular hypothalamic nu., locas coeruleus, and nucleus tractus solitarious. The results could interpreted to mean that the limbic, hypothalamus, and brainstem system is activated in response to extensive gut necrosis and participate in neuroendocrine, autonomic, and emotional change. Moreover, it is suggested that attenuated c-Jun IR in the hippocampal dentate gyrus at 8 h after SMAO may be neuroanatomical evidence of memory disturbance in patients with severe abdominal sepsis.

[背景]（1）脓毒症是近年来公认的一种全身炎症反应综合征（systemic inflammatory response syndrome，SIRS），主要表现为体温、心率、呼吸频率和白细胞计数的改变。这些表现清楚地表明，在这种情况下，体液和神经元的调节深深参与宿主的反应。因此，深入了解中枢神经系统（CNS）的变化是有价值的，以进一步了解脓毒症的病理生理学。(2)c-Fos和c-Jun分别是由c-fos和c-jun编码的诱导型转录因子（ITFs）。c-Jun的转录活性被广泛用作激活神经元的代谢标志物。[目的]使用抗c-Fos、c-Jun和磷酸化c-Jun的抗体进行研究以确定SIRS状态如何影响CNS。[结果与讨论] SMAO后8h大鼠脑组织免疫组化染色显示c-Fos、c-Jun和p-c-Jun免疫反应阳性细胞主要分布于杏仁中央核和基底外侧核。下丘脑室旁核，蓝斑和孤束核。这些结果可以解释为边缘系统、下丘脑和脑干系统响应广泛的肠坏死而被激活，并参与神经内分泌、自主神经和情绪变化。SMAO后8 h海马齿状回c-Jun免疫反应减弱可能是严重腹腔脓毒症患者记忆障碍的神经解剖学证据。