A study on mechanisms of odontoblast differentiation during reparative dentinogenesis.

修复牙本质发生过程中成牙本质细胞分化机制的研究。

• 批准号: 10671789
• 负责人: YOSHIBA Kunihiko
• 金额: $ 2.05万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671789/
• 关键词: Reparative dentinogenesis; Odontoblasts; Basement membrane; Extracellular matrix; Dental caries; Cavity preparation; Antigen presenting cell; Immunohistochemistry; 歯牙移植; 歯髄内石灰化; 非コラーゲン蛋白; ラミニン-5; インテグリン; Er:YAGレーザー; 上皮-間葉相互作用; 神経線維

In order to elucidate mechanisms by which pulp cells differentiate into odontoblasts during reparative dentinogenesis, immunohistochemical analyses for several factors related to cell differentiation were performed. The results were as follows :1. During tooth development, differential expression of laminin-5 and integrin α6 and β4 subunits in the inner dental epithelium may be involved in ameloblast differentiation. These changes in basement membrane composition might be also related to odontoblast differentiation.2. In pulps affected by early caries, MHC class II antigen-expressing cells (HLA-DR-positive dendritic cells) aggregated mainly in the cell-free zone associated with PGP 9.5-immuno-reactive nerve fibers. In advanced caries, the accumulated HLA-DR-positive cells and PGP 9.5-immunoreactive nerve fibers showed close association with each other especially beneath the odontoblast layer. Class II molecules were recognized not only in dendritic cells but also in the Schwann cells o … More f non-myelinated nerves in the pulp. These suggested a synergistic action between immune and nervous systems in pulpal inflammation and reparative process.3. Pulpal responses after cavity preparation with either Er : YAG laser or a conventional drill were evaluated. The immunoreactivity for tissue non-specific alkaline phosphatase was more pronounced in the laser group. Clear similarities in the distribution patterns of OX6-immunopositive cells and PGP 9.5- immunoreactive nerve fibers were noted. OX6-positive MHC class II antigen-expressing cells accumulated along the pulp-dentin border and numerous bead-like PGP 9.5-immunoreactive nerve fibers were observed under the odontoblastic layer, suggesting their involvement in pulp tissue repair and reparative dentin formation.4. During hard tissue formation in the pulp cavity of rat molar after transplantation into subcutaneous tissue, non-collagenous proteins, such as osteocalcin, osteopontin, bone sialoprotein and dentin sialoprotein showed different distribution patterns in newly-formed hard tissues in the coronal, root canal and apex areas, respectively. These suggested that each hard tissue was formed by differently-derived cells. Less

为了阐明牙髓细胞在修复性牙本质形成过程中分化为成牙本质细胞的机制，对与细胞分化相关的几个因素进行了免疫组织化学分析。结果如下：1.在牙齿发育过程中，层粘连蛋白-5和整合素α-6、β-4亚基在内牙上皮细胞中的差异表达可能参与成釉细胞的分化。这些基底膜成分的改变也可能与成牙本质细胞分化有关。在受早期龋病影响的牙髓中，表达MHC-II类抗原的细胞(HLA-DR阳性的树突状细胞)主要聚集在与PGP9.5免疫反应神经纤维相关的无细胞区域。晚期龋组中，堆积的HLADR阳性细胞与PGP9.5阳性神经纤维密切相关，尤其是在成牙本质细胞层下。II类分子不仅在树突状细胞中被识别，而且在…的雪旺细胞中也被识别。牙髓中有更多的非髓鞘神经。提示免疫系统和神经系统在牙髓炎症和修复过程中具有协同作用。用Er：YAG激光和常规钻头制备洞洞后，评估牙髓的反应。组织非特异性碱性磷酸酶免疫反应在激光组更明显。OX6免疫阳性细胞和PGP9.5免疫反应神经纤维的分布模式明显相似。OX6阳性的MHC-II类抗原表达细胞聚集在牙髓-牙本质交界处，成牙本质细胞层下可见大量珠状PGP9.5免疫反应神经纤维，提示它们参与了牙髓组织修复和修复性牙本质的形成。大鼠磨牙皮下移植后牙髓腔内硬组织形成过程中，骨钙素、骨桥蛋白、骨涎蛋白和牙本质唾液蛋白等非胶原蛋白在牙冠、根管和根尖区的新生硬组织中表现出不同的分布模式。这表明，每个硬组织都是由不同来源的细胞形成的。较少

项目成果

Nagako Yoshiba: "Immunohistochemical localization of class II antigens and nerve fibers in human carious teeth: HLA-DR immunoreactivity in Schwann cells." Archives of Histology and Cytology. 61(4). 343-352 (1998)

Nagako Yoshiba：“人类龋齿中 II 类抗原和神经纤维的免疫组织化学定位：雪旺细胞中的 HLA-DR 免疫反应性。”

Nagako Yoshiba et al.: "Expression and localization of laminin-5 subunits in the mouse incisor."Cell and Tissue Research. 292(1). 143-149 (1998)

Nagako Yoshiba 等人：“层粘连蛋白 5 亚基在小鼠门牙中的表达和定位。”细胞和组织研究。

Kunihiko Yoshiba: "Expression and localization of laminin-5 subunits during mouse tooth development." Developmental Dynamics. 211. 164-176 (1998)

Kunihiko Yoshiba：“小鼠牙齿发育过程中层粘连蛋白 5 亚基的表达和定位。”

Nagako Yoshiba: "Immunohistochemical localization of class II antigens and nerve fibers in human carious teeth : HLA-DR immunoreactivity in Schwann cells."Archives of Histology and Cytology. 61. 343-352 (1998)

Nagako Yoshiba：“人类龋齿中 II 类抗原和神经纤维的免疫组织化学定位：雪旺细胞中的 HLA-DR 免疫反应性。”组织学和细胞学档案。

Kunihiko Yoshiba: "Differential expression of laminin-5 subunits during incisor and molar development in the mouse."International Journal of Developmental Biology. 44. 337-340 (2000)

Kunihiko Yoshiba：“小鼠门牙和磨牙发育过程中层粘连蛋白 5 亚基的差异表达。”国际发育生物学杂志。