A study on mechanisms of odontoblast differentiation during reparative dentinogenesis.

修复牙本质发生过程中成牙本质细胞分化机制的研究。

基本信息

批准号：
10671789
负责人：
YOSHIBA Kunihiko
金额：
$ 2.05万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

In order to elucidate mechanisms by which pulp cells differentiate into odontoblasts during reparative dentinogenesis, immunohistochemical analyses for several factors related to cell differentiation were performed. The results were as follows :1. During tooth development, differential expression of laminin-5 and integrin α6 and β4 subunits in the inner dental epithelium may be involved in ameloblast differentiation. These changes in basement membrane composition might be also related to odontoblast differentiation.2. In pulps affected by early caries, MHC class II antigen-expressing cells (HLA-DR-positive dendritic cells) aggregated mainly in the cell-free zone associated with PGP 9.5-immuno-reactive nerve fibers. In advanced caries, the accumulated HLA-DR-positive cells and PGP 9.5-immunoreactive nerve fibers showed close association with each other especially beneath the odontoblast layer. Class II molecules were recognized not only in dendritic cells but also in the Schwann cells o … More f non-myelinated nerves in the pulp. These suggested a synergistic action between immune and nervous systems in pulpal inflammation and reparative process.3. Pulpal responses after cavity preparation with either Er : YAG laser or a conventional drill were evaluated. The immunoreactivity for tissue non-specific alkaline phosphatase was more pronounced in the laser group. Clear similarities in the distribution patterns of OX6-immunopositive cells and PGP 9.5- immunoreactive nerve fibers were noted. OX6-positive MHC class II antigen-expressing cells accumulated along the pulp-dentin border and numerous bead-like PGP 9.5-immunoreactive nerve fibers were observed under the odontoblastic layer, suggesting their involvement in pulp tissue repair and reparative dentin formation.4. During hard tissue formation in the pulp cavity of rat molar after transplantation into subcutaneous tissue, non-collagenous proteins, such as osteocalcin, osteopontin, bone sialoprotein and dentin sialoprotein showed different distribution patterns in newly-formed hard tissues in the coronal, root canal and apex areas, respectively. These suggested that each hard tissue was formed by differently-derived cells. Less

为了阐明牙髓细胞在修复性牙本质发生过程中分化为牙糖细胞的机制，对与细胞分化有关的几个因素进行了免疫组织化学分析。结果如下：1。在牙齿发育过程中，内部牙齿上皮中层粘连蛋白5和整联蛋白α6和β4亚基的差异表达可能参与木质细胞分化。基底膜组成中的这些变化也可能与牙植物分化有关。2。在受早期携带影响的浆中，MHC II类表达抗原的细胞（HLA-DR阳性树突状细胞）主要集合在与PGP 9.5免疫反应神经纤维相关的无细胞区域中。在高级汽车中，累积的HLA-DR阳性细胞和PGP 9.5-免疫反应性神经纤维彼此之间彼此紧密相关，尤其是在Odontoblast层下方。 II类分子不仅在树突状细胞中被识别，而且在Schwann细胞中也识别出果肉中更多的非髓鞘神经。这些表明免疫系统和神经系统在牙髓感染和修复过程中的协同作用3。用ER：YAG激光或常规钻头进行腔体准备后的牙髓反应。在激光组中，组织非特异性酒精磷酸酶的免疫反应性更为明显。注意到OX6-免疫阳性细胞和PGP 9.5-免疫反应性神经纤维的分布模式的明显相似性。 OX6阳性MHC II类表达抗原抗原的细胞沿浆丁丁丁属边界积累，并且在odontodolbastic层下观察到了许多珠子样PGP 9.5-免疫反应性神经纤维，这表明它们参与了纸浆组织修复和重复性牙本质的形成。4。在将大鼠磨牙的果肉腔中的硬组织形成中，将非胶原蛋白（例如骨钙素，骨opontin，骨pototin，sialoponin和dentin sialopon和dentin sialoptin）在殖民地，根cal和Epex中的新成型硬组织中表现出不同的分布模式，相应地相应。这些表明每个硬组织都是由不同衍生的细胞形成的。较少的