A study on mechanisms of tissue formation and regeneration of the dentin-pulp complex

牙本质-牙髓复合体组织形成与再生机制的研究

基本信息

批准号：
16591910
负责人：
YOSHIBA Kunihiko
金额：
$ 2.3万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

This study aims to elucidate mechanisms of tissue formation and regeneration of the dentin-pulp complex. The results were as follows :1. During mouse tooth development, the expression of an intermediate filament nestin was intense in differentiating odontoblasts, and became restricted to the secretory pole of functional odontoblasts, suggesting that nestin plays a role in terminal differentiation, especially in cell elongation and/or polarization of odontoblasts.2. Distinct temporal and spatial expression patterns of TIMPs suggest divergent functions of these factors in incisor organogenesis. Intense expression of Timp-1 was detected in differentiating odontoblasts, while Timp-3 was expressed by subodontoblasts, suggesting their involvement in odontoblast differentiation.3. The unerupted rat incisor was composed of osteodentin, with numerous cells present in the anterior apex. The osteodentin was immunopositive for osteocalcin, bone sialoprotein, and dentin sialoprotein, with an immunolocalization pattern similar to that of dentin. These results indicate that osteodentin in the rat incisor possesses a dentin-like characteristics.4. We performed a histological and immunohistochemical evaluation of mineralized tissue induced by pulp transplantation into subcutaneous tissue. The results indicate that pulp cells are able to form hard tissue with bone- or cementum-like characteristics without dentin influence. Calcification may start in necrotic cells and matrix vesicles, followed by collagenous calcification.5. Pulpal responses to GaAlAs laser irradiation were elucidated. The results indicated that the GaAlAs laser may induce the formation of tertiary dentin by influencing the secretory activity of odontoblasts. However, higher energies may cause irreversible changes to the pulp, often leading to the formation of an intrapulpal bone-like tissue.

这项研究旨在阐明组织形成和牙本质-Pulp复合物再生的机制。结果如下：1。在小鼠牙齿发育过程中，中间丝巢的表达在分化的Odontobasts中很强烈，并仅限于功能性odontotoblasts的分泌极，这表明Nestin在末端分化中起作用，尤其是在细胞伸长和/或Odontoblasts.2。 TIMP的不同时间和空间表达模式表明这些因素在门牙器官发生中的功能不同。 TIMP-1的强烈表达在分化的Odontoblasts中检测到，而TIMP-3则通过亚额叶细胞表达，这表明它们参与了odontoblast分化。3。联合爆发的大鼠门牙由骨丁蛋白组成，前端有许多细胞。骨毒素对骨钙素，唾液蛋白和乙蛋白苏蛋白的免疫阳性，具有与牙本质相似的免疫定位模式。这些结果表明，大鼠门牙中的骨素具有类似牙本质的特征。4。我们对通过移植到皮下组织诱导的矿化组织进行了组织学和免疫组织化学评估。结果表明，纸浆细胞能够形成具有骨骼或骨骼样特征的硬组织，而不会影响牙本质。钙化可能从坏死细胞和基质囊泡开始，然后进行胶原钙化5。阐明了对Gaalas激光照射的牙髓反应。结果表明，Gaalas激光器可以通过影响Odontoblasts的分泌活性来诱导三级牙本质的形成。但是，较高的能量可能会导致果肉的不可逆转变化，通常导致形成毛内骨状组织。

项目成果

期刊论文数量（24）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Class II antigen-presenting dendritic cell and nerve fiber responses to cavities, caries, or caries treatment in human teeth.

II类抗原呈递树突状细胞和神经纤维对人类牙齿中的蛀牙、龋齿或龋齿治疗的反应。

DOI：
发表时间：
2004
期刊：
Clinical Research in Dentistry 1(2)
影响因子：
0
作者：
金澤紀子;米山武義;植田耕一郎;足立三枝子編;Ohba T.;Kunihiko Yoshiba et al.
通讯作者：
Kunihiko Yoshiba et al.

ヒトの歯における窩洞形成,う蝕,ならびにう蝕治療に対するクラスII抗原提示樹状細胞と神経線維の反応

II类抗原呈递树突状细胞和神经纤维对人类牙齿空洞形成、龋齿和龋齿治疗的反应。

DOI：
发表时间：
2004
期刊：
歯科臨床研究 1(2)
影响因子：
0
作者：
Ohara N;Hayashi Y;Yamada S;Kim S-K;Matsunaga T;Yanagiguchi K;Ikeda T;Kunihiko Yoshiba;Morimoto Y.;T.Suge et al.;M.Sawajiri;Ozaki A.;吉羽邦彦
通讯作者：
吉羽邦彦

Immune defense mechanisms of the dentin-pulp complex - Response of pulpal dendritic cells to caries, cavity preparation and restoration.

牙本质-牙髓复合体的免疫防御机制 - 牙髓树突状细胞对龋齿、空腔准备和修复的反应。

DOI：
发表时间：
2005
期刊：
the Quintessence 24(8)
影响因子：
0
作者：
Ohara N;Hayashi Y;Yamada S;Kim S-K;Matsunaga T;Yanagiguchi K;Ikeda T;Kunihiko Yoshiba
通讯作者：
Kunihiko Yoshiba

象牙質/歯髄複合体の生体防御機構う蝕,窩洞形成,修復処置に対する歯髄内樹状細胞の応答

牙本质/牙髓复合体的生物防御机制牙髓中的树突状细胞对龋齿、空洞形成和修复程序的反应