A study on mechanisms of tissue formation and regeneration of the dentin-pulp complex

牙本质-牙髓复合体组织形成与再生机制的研究

• 批准号: 16591910
• 负责人: YOSHIBA Kunihiko
• 金额: $ 2.3万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591910/
• 关键词: dentin-pulp complex; regeneration therapy; odontoblasts; nestin; TIMPs; non-collagenous proteins; dentin sialoprotein; laser; 非コラーゲン性タンパク; 骨様象牙質; 修復象牙質; 樹状細胞

This study aims to elucidate mechanisms of tissue formation and regeneration of the dentin-pulp complex. The results were as follows :1. During mouse tooth development, the expression of an intermediate filament nestin was intense in differentiating odontoblasts, and became restricted to the secretory pole of functional odontoblasts, suggesting that nestin plays a role in terminal differentiation, especially in cell elongation and/or polarization of odontoblasts.2. Distinct temporal and spatial expression patterns of TIMPs suggest divergent functions of these factors in incisor organogenesis. Intense expression of Timp-1 was detected in differentiating odontoblasts, while Timp-3 was expressed by subodontoblasts, suggesting their involvement in odontoblast differentiation.3. The unerupted rat incisor was composed of osteodentin, with numerous cells present in the anterior apex. The osteodentin was immunopositive for osteocalcin, bone sialoprotein, and dentin sialoprotein, with an immunolocalization pattern similar to that of dentin. These results indicate that osteodentin in the rat incisor possesses a dentin-like characteristics.4. We performed a histological and immunohistochemical evaluation of mineralized tissue induced by pulp transplantation into subcutaneous tissue. The results indicate that pulp cells are able to form hard tissue with bone- or cementum-like characteristics without dentin influence. Calcification may start in necrotic cells and matrix vesicles, followed by collagenous calcification.5. Pulpal responses to GaAlAs laser irradiation were elucidated. The results indicated that the GaAlAs laser may induce the formation of tertiary dentin by influencing the secretory activity of odontoblasts. However, higher energies may cause irreversible changes to the pulp, often leading to the formation of an intrapulpal bone-like tissue.

本研究旨在阐明牙本质-牙髓复合体的组织形成和再生机制。结果如下：1.在小鼠牙齿发育过程中，中间丝Nestin在成牙本质细胞分化过程中表达强烈，并局限于功能性成牙本质细胞的分泌极，提示Nestin在成牙本质细胞的终末分化，尤其是细胞伸长和/或极化过程中起重要作用。TIMPs不同的时间和空间表达模式提示这些因子在切牙器官发生中的不同功能。TIMP-1在成牙本质细胞分化过程中表达较强，而TIMP-3在亚成牙本质细胞中表达，提示其参与成牙本质细胞的分化。未萌出的大鼠切牙由成骨蛋白组成，在前牙尖部有大量细胞。骨钙素、骨涎蛋白和牙本质唾液蛋白免疫阳性，免疫定位模式与牙本质相似。这些结果表明，大鼠切牙中的骨质素具有牙本质样特征。我们对牙髓移植到皮下组织诱导的矿化组织进行了组织学和免疫组织化学评价。结果表明，牙髓细胞能够在不影响牙本质的情况下形成具有骨样或牙骨质样特征的硬组织。钙化可能始于坏死性细胞和基质小泡，随后是胶原性钙化。阐明了牙髓对GaAlAs激光照射的反应。结果提示，GaAlAs激光可能通过影响成牙本质细胞的分泌活性而诱导三级牙本质的形成。然而，较高的能量可能会对牙髓造成不可逆转的变化，通常会导致牙髓内骨样组织的形成。

项目成果

Odontoblast responses to GaAlAs laser irradiation in rat molars: an experimental study using heat-shock protein-25 immunohistochemistry

• DOI: 10.1111/j.1600-0722.2006.00261.x
• 发表时间: 2006-02-01
• 期刊: EUROPEAN JOURNAL OF ORAL SCIENCES
• 影响因子: 1.9
• 作者: Tate, Y;Yoshiba, K;Ohshima, H
• 通讯作者: Ohshima, H

Differential regulation of TIMP-1,-2, and-3 mRNA and protein expressions during mouse incisor development

小鼠门牙发育过程中TIMP-1、-2和-3 mRNA和蛋白表达的差异调节

• 发表时间: 2006
• 期刊: Cell and Tissue Research 324
• 作者: Yoshiba N;Yoshiba K;Stoetzel C;Perrin-Schmitt F;Cam Y;Ruch JV;Hosoya A;Ozawa H;Lesot H.
• 通讯作者: Lesot H.

Class II antigen-presenting dendritic cell and nerve fiber responses to cavities, caries, or caries treatment in human teeth.

II类抗原呈递树突状细胞和神经纤维对人类牙齿中的蛀牙、龋齿或龋齿治疗的反应。

• 发表时间: 2004
• 期刊: Clinical Research in Dentistry 1(2)
• 作者: 金澤紀子;米山武義;植田耕一郎;足立三枝子編;Ohba T.;Kunihiko Yoshiba et al.
• 通讯作者: Kunihiko Yoshiba et al.

ヒトの歯における窩洞形成,う蝕,ならびにう蝕治療に対するクラスII抗原提示樹状細胞と神経線維の反応

II类抗原呈递树突状细胞和神经纤维对人类牙齿空洞形成、龋齿和龋齿治疗的反应。

• 发表时间: 2004
• 期刊: 歯科臨床研究 1(2)
• 作者: Ohara N;Hayashi Y;Yamada S;Kim S-K;Matsunaga T;Yanagiguchi K;Ikeda T;Kunihiko Yoshiba;Morimoto Y.;T.Suge et al.;M.Sawajiri;Ozaki A.;吉羽邦彦
• 通讯作者: 吉羽邦彦

Immune defense mechanisms of the dentin-pulp complex - Response of pulpal dendritic cells to caries, cavity preparation and restoration.

牙本质-牙髓复合体的免疫防御机制 - 牙髓树突状细胞对龋齿、空腔准备和修复的反应。

• 发表时间: 2005
• 期刊: the Quintessence 24(8)
• 作者: Ohara N;Hayashi Y;Yamada S;Kim S-K;Matsunaga T;Yanagiguchi K;Ikeda T;Kunihiko Yoshiba
• 通讯作者: Kunihiko Yoshiba