Study on the three dimensional calcification around the surface of biomaterial granules using bovine osteoblastic primary culture

本研究的目的是利用成骨细胞原代培养技术研究几种生物材料表面三维成骨的情况。然而，由于成骨细胞原代培养的一些限制，包括胎牛骨细胞，我刚刚发现猪胚胎下颌骨成骨原代培养在某些情况下会出现基质钙化。当商业上可用的人骨成骨原代培养物加羟基磷灰石颗粒培养时，它显示出二维培养中没有的有趣结构。这意味着成骨细胞原代培养可能是研究与生物材料颗粒共培养时体内三维成骨机制的有力工具。然而，最大的问题是这些细胞的增殖和分化非常缓慢，活性太低。现在，我们开发了一种新的猪下颌骨成骨细胞原代培养方法。该培养体系在某些情况下表现出周围基质的快速钙化。基质快速钙化的最佳条件仍需阐明。

The aim of this study was to investigate on three dimensional bone formations on the surface of several biomaterials using osteoblastic primary culture. However, because of several restrictions regarding osteoblastic primary culture, including fetal bovine bone cells, I've just found that osteoblastic primary culture derived from embryonic porcine mandibular bone shows matrix calcifications under some circumstances so far.When commercially available osteoblastic primary culture derived from human bone was cultured with hydroxyl apatite granules, it showed interesting structures that was not seen in the two dimensional cultures. This means osteoblastic primary cultures may be powerful tools to study the mechanisms of three dimensional bone formations in vivo when co-cultured with biomaterial granules. However, the biggest problem was that proliferation and differentiation of those cells were very slow and the viability of those was too low.Now, a new osteoblastic primary culture derived from embryonic porcine mandibular bone was developed. This culture system showed rapid calcification among surrounding matrix under some circumstances. Elucidation of the optimal conditions for rapid calcification of matrix still needs to be done.