Study on the three dimensional calcification around the surface of biomaterial granules using bovine osteoblastic primary culture

牛成骨细胞原代培养生物材料颗粒表面三维钙化研究

• 批准号: 10671817
• 负责人: UOSHIMA Katsumi
• 金额: $ 2.37万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671817/
• 关键词: Biomaterial Granule; Osteoblast; Calcification

The aim of this study was to investigate on three dimensional bone formations on the surface of several biomaterials using osteoblastic primary culture. However, because of several restrictions regarding osteoblastic primary culture, including fetal bovine bone cells, I've just found that osteoblastic primary culture derived from embryonic porcine mandibular bone shows matrix calcifications under some circumstances so far.When commercially available osteoblastic primary culture derived from human bone was cultured with hydroxyl apatite granules, it showed interesting structures that was not seen in the two dimensional cultures. This means osteoblastic primary cultures may be powerful tools to study the mechanisms of three dimensional bone formations in vivo when co-cultured with biomaterial granules. However, the biggest problem was that proliferation and differentiation of those cells were very slow and the viability of those was too low.Now, a new osteoblastic primary culture derived from embryonic porcine mandibular bone was developed. This culture system showed rapid calcification among surrounding matrix under some circumstances. Elucidation of the optimal conditions for rapid calcification of matrix still needs to be done.

这项研究的目的是使用成骨细胞原代培养物研究几个生物材料表面的三维骨形成。但是，由于对成骨细胞原发性培养（包括胎牛骨细胞）的几个限制，我刚刚发现，源自胚胎猪下颌骨的成骨细胞原发性培养在到目前为止的某些情况下显示出基质的钙化。在到目前为止，在迄今为止，在远处可用的成骨细胞培养的人类骨骼培养了两种培养物，这些培养物与Hydroxyl apate Granens contenture contractrastion cormenters cormenture cortine conteciption。文化。这意味着成骨细胞原代培养物可能是研究与生物材料颗粒共培养时体内三维骨形成的机制的强大工具。但是，最大的问题是这些细胞的增殖和分化非常慢，并且这些细胞的生存能力太低了。现在，开发了一种从胚胎猪下颌骨骼中得出的新的成骨细胞原发性培养。在某些情况下，该培养系统显示出周围矩阵的钙化快速钙化。仍需要阐明快速钙化矩阵的最佳条件。