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Fabrication and histological evaluation of bone-replacing calcium phosphate cement using NaHCOィイD23ィエD2

NaHCO2D23 骨替代磷酸钙骨水泥的制备及组织学评价

基本信息

批准号：
10671885
负责人：
NAKANISHI Hiroaki
金额：
$ 2.05万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671885/
关键词：
apatite cement crystallinity bone-replacing NaHCO3 osteoclast アパタイト骨置換型

项目摘要

Alternation of a composition of set apatite cement (AC) from carbonate-free apatite (CO3-free AP) to carbonate apatite (CO3-AP) may accelerate replacement of the set AC with bone since CO3-AP can be dissolved much faster than CO3-free AP in the weak acidic solution produced by osteoclast. In this investigation, NaHCO3 was added to the AC component and the effects of added NaHCO3 on cement setting behavior and on set mass were studied as an initial step for the fabrication of AC which can be replaced with bone faster than current AC. When NaHCO3 was added to AC, the resultant set mass contained CO3. Although not all CO3 was incorporated in the set AC, the amount of CO3 incorporated into the set AC increased with the amount of added NaHCO3. FT-IR measurement revealed the formation of B-type CO3-AP. The setting time was prolonged from 30 min to 45 min, indicating that NaHCO3 has an inhibitory effect on AP formation. The diametral tensile strength (DTS) of the set AC decreased significantly with the addition of NaHCO3. Scanning electron microscopic observation revealed that fine crystals were formed in the set AC when NaHCO3 was added to AC. As a result, the crystallinity indexes of the set AC measured using powder x-ray diffraction (XRD) and FT-IR decreased with an increase in the amount of added NaHCO3. The dissolution rate of set AC in weak acid, pH 5.5, increased with the amount of added NaHCO3. The number of resorption pits and the resorbed area by cultured osteoclasts increased according to added CO3 content. The resorbed area on 4% CO3 containing-AC was twice as much as carbonate-free AC. The results suggested that CO3-AP-formed AC would be easily resorbed by osteoclasts and have good potential value for its use in wider clinical applications in oral and maxillofacial, plastic and reconstructive surgery.

从无碳酸盐磷灰石（CO3-free AP）到碳酸盐磷灰石（CO3-AP）的组合交替可能会加速固定的AC与骨的替换，因为在破骨细胞产生的弱酸性溶液中，CO3-AP比无co3 AP溶解得快得多。在本研究中，将NaHCO3添加到AC组分中，并研究了添加的NaHCO3对水泥凝结行为和固化质量的影响，作为制造比现有AC更快地被骨取代的AC的第一步。当NaHCO3添加到AC中时，得到的固化质量含有CO3。虽然并不是所有的CO3都被纳入到固定AC中，但CO3的纳入量随着NaHCO3的加入量的增加而增加。FT-IR测定显示b型CO3-AP的形成。凝固时间由30 min延长至45 min，说明NaHCO3对AP的形成有抑制作用。NaHCO3的加入显著降低了复合材料的直径抗拉强度（DTS）。扫描电镜观察发现，在AC中加入NaHCO3后，在set AC中形成了细小的晶体。因此，随着NaHCO3加入量的增加，使用粉末x射线衍射（XRD）和FT-IR测量的set AC的结晶度指标降低。NaHCO3在弱酸（pH 5.5）中的溶解速率随NaHCO3加入量的增加而增加。随着CO3含量的增加，体外培养的破骨细胞的吸收坑数量和吸收面积增加。含4% CO3- AC的AC的吸收面积是无碳酸盐AC的2倍。结果表明，CO3- ap形成的AC易于被破骨细胞吸收，在口腔颌面外科、整形外科和重建外科的临床应用中具有良好的潜在价值。