Effects of joint movent or joint immobilization on experimental heterotopic ossification

关节运动或关节固定对实验性异位骨化的影响

• 批准号: 10838015
• 负责人: KAWAMURA Morio
• 金额: $ 0.58万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10838015/
• 关键词: Experimental heterotopic ossification; Bone morphogenetic protein; passive movement of joints; joint immobilization

The purpose of this study is to investigate effects of forced passive joint movement or joint immobilization on the experimental heterotopic ossification which is induced by bone morphogenetic protein implanted in murine quadriceps muscle. The crude bone morphogenetic protein was extracted from the bovine cortical bone as a fraction of 4M-GuHClsoluble, 0.5M-GuHCl insoluble and water insoluble. The total amount of protein powder weighed up to 1647mg after lyophilization. This protein showed active and stable new bone formation in murine muscle by implantation. Ten milligram of protein powder was implanted in murine quadriceps muscle and each mouse was devided into three groups. The first movement/immobilized group were imposed passive movement on the knee and the hip joint for 20 minutes with CPM machine and then immobilized from the hip through the ankle with plaster cast everyday. The second group was only immobilized as same. The third group was kept under free movement. Three weeks after implantation, the new bone formation was confirmed in all of murine thigh muscle with softex film. The new bone was sampled out and sintered at 1000℃. The ash weight was 2.95mg in the 1st group, 1.98mg in the 2nd, and 2.12mg in the 3rd. The 1st group showed higher bone formation than the other groups. Pathohistologically, myotube cells appeared around the protein powder at the early stage of implantation in each group, which seems to be dedifferentiation of surroundings mesenchymal tissue. Fourteen days of implantation, the 1st group tended to show small hemorrhage and vascular proliferation in the surrounding fibrous tissue compared with other groups. In the next, early phase (first 7days), intermediate phase (2nd 7days) and late phase (3rd 7days) of passive movement was applied on the movement /immobilized group, but there was no difference of new bone formation among these three groups.

本研究旨在探讨强迫被动关节运动或关节制动对骨形态发生蛋白植入小鼠股四头肌诱导的实验性异位骨化的影响。从牛皮质骨中提取的粗骨形态发生蛋白分为4 M-GuHCl可溶性、0. 5 M-GuHCl不溶性和水不溶性组分。冻干后蛋白粉总量达1647 mg。该蛋白在小鼠肌肉中通过植入显示活跃且稳定的新骨形成。将10 mg蛋白粉植入小鼠股四头肌，每只小鼠分为三组。第一运动/制动组每天在CPM机上进行膝、髋关节被动运动20 min，然后用石膏管型从髋关节经踝关节进行制动。第二组也是一样。第三组保持自由流动。植入后3周，用softex胶片证实小鼠大腿肌肉中全部新骨形成。取新骨，1000℃烧结。灰重第1组为2.95mg，第2组为1.98mg，第3组为2.12mg。第1组显示出比其他组更高的骨形成。病理组织学上，各组植入早期蛋白粉周围均出现肌管细胞，可能是周围间充质组织的去分化。植入14天后，与其他组相比，第1组倾向于显示周围纤维组织中的小出血和血管增生。运动/制动组分别进行早期（前7 d）、中期（后7 d）和晚期（后7 d）被动运动，但三组间新骨形成无差异。

项目成果

伊藤真理子,北澤佐和枝,河村守雄: "実験的異所性骨化に対する強制他動運動と不動化の影響" 名古屋大学医療技術短期大学部紀要. 第9巻. 37-42 (1997)

Mariko Ito、Sawae Kitazawa、Morio Kawamura：“强迫被动运动和固定对实验性异位骨化的影响”名古屋大学医学技术系通报，第 9 卷，37-42 (1997)

ITO,M., KAWAMURA,M., et al.: "Effect of Forced Passive Movement and Immobilization on Experimental Heterotopic Ossification."Bulletin of Nagoya University College of Medical Technology. 9. 37-42 (1997)

ITO,M.、KAWAMURA,M. 等人：“强制被动运动和固定对实验性异位骨化的影响”。名古屋大学医学技术学院通报。