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Effects of joint movement or joint immobilization on experimental heterotopic ossification

关节运动或关节固定对实验性异位骨化的影响

基本信息

批准号：
12832022
负责人：
KAWAMURA Morio
金额：
$ 1.09万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12832022/
关键词：
Experimental heterotopic ossification Bone morphogenetic protein passive movement of joints timing of forced movement pathohistological study 間接他動運動強制他動運動関節不動化

项目摘要

The purpose of this study is to investigate the relationship between forced passive joint movement and experimental heterotopic ossification which is induced by bone morphogenetic protein (BMP) implanted in murine quadriceps muscle. The crude bone morphogenetic protein was extracted from the bovine cortical bone as a fraction of 4M-GuHCl soluble, 0.5M-GuHCl insoluble and water insoluble. The total amount of protein powder weighed up to 800mg after lyophilization. This protein showed active and stable new bone formation in murine muscle by implantation. Ten miligram of protein powder was implanted in each murine quadriceps muscle.In the present study, the effect of forced passive movement on heterotopic ossification was examined in terms of application phase of passive movement and pathological findings. Three weeks after BMP implantation, the new bone formation was comfirmed in all of murine thigh muscle with softex film. The new bone was sampled out and sintered at 1000℃ and ash weight was measured. As a result, the most amounts of new bone formation was obtaind in early phase group mice in which the passive movement was applied for the first week, late phase group (the 3rd week's application) showed the less amount of bone formation (p<0.05), and the middle phase (the 2nd week's application) showed the least bone formation. In the pathological study, the application phase of movement affected on the locational stability of BMP material in the surrounding tissue. The early phase group showed low BMP stability and resulted in wide range of ossification and low maturity of new bone.

本研究旨在探讨强迫被动关节运动与骨形态发生蛋白（BMP）植入小鼠股四头肌诱导异位骨化的关系。从牛皮质骨中提取的粗骨形态发生蛋白分为4 M-GuHCl可溶性、0. 5 M-GuHCl不溶性和水不溶性组分。冻干后蛋白粉总量达到800 mg。该蛋白在小鼠肌肉中通过植入显示活跃且稳定的新骨形成。将10毫克蛋白粉植入小鼠股四头肌，观察被动运动对异位骨化的影响，并与病理结果进行比较。BMP植入3周后，软X线片显示小鼠大腿肌肉均有新骨形成。取新骨，1000℃烧结，测定灰重。结果表明，被动运动第1周的早期组小鼠新骨形成量最多，晚期组（第3周）骨形成量最少（p<0.05），中期组（第2周）骨形成量最少。在病理学研究中，运动的施加时相影响BMP材料在周围组织中的定位稳定性。早期组BMP稳定性差，成骨范围广，新骨成熟度低。