糖鎖による超分子中の水の運動性及び水素結合特性の磁気共鳴法による検出と応用研究

超分子糖链中水的迁移率和氢键性质的磁共振方法检测及应用研究

基本信息

批准号：
11640605
负责人：
WATANABE Tokuko
金额：
$ 2.18万
依托单位：
Tokyo University of Fisheries
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

1. Construction of the detection system for evaluating the dynamics and interactions of water and polysaccharides : (1) Solid echo method for detecting very short T2 (>l0μs) was combined to the usually used CPMG method to evaluate the amount of frozen water (originally free water) and un-frozen water (a portion of hydration water), differentiation between the inert protons and the labile protons in the polymer chain, and hydration properties of polysaccharides. (2) System for detecting slow motion of polymer and hydration water was constructed employing high field gradient for diffusion measurement due to the pulsed field gradient SE or STE and/or NMR microimaging for visualization. (3) Simultaneous analyzing system of T2 and Diffusion coefficient : Pulse sequence for diffusion measurement was modified to keep the fractions of species in samples constant. (4) Theoretical treatment of temperature dependence of T2 in the presence of a chemical proton exchange and a diffusive molecular exchange : differential equations for time dependence of magnetization due to water proton were numerically analyzed to obtain possible change of T2 with the temperature change under the experimental conditions.2. Water mobility in concentrated pullulan solutions : By using the established systenn 1 (1) and(3), frozen water (T2 10μs), unfrozen water (T2 100μs) and inert polymer protons (T2 1ms) at -11℃ were distinguished each other and in the temperature more than 0℃ hydration water and free water was not differentiate by rapid diffusive molecular exchange. The flexibility of pullulan chain in aqueous solution is proved.3. Uptake mechanism of divalent cation by gellan gum was analyzed by Cu(II) EPR method and the mechanisn was made clear in the molecular level.4. Kinetic studies of the gelation process of gelatin was investigated based on ^1H T_2 NMR, CD and viscoelesticity measurements. Hydrogen bonding dormation in prior to the coil formation was proved.

1. Construction of the detection system for evaluating the dynamics and interactions of water and polysaccharides: (1) Solid echo method for detecting very short T2 (>l0μs) was combined to the usually used CPMG method to evaluate the amount of frozen water (originally free water) and un-frozen water (a portion of hydration water), differentiation between the inert protons and the labile protons in the polymer chain, and多糖的水合特性。（2）用于检测聚合物和水合水慢运动的系统，该系统使用高场梯度进行了高场梯度进行扩散测量，这是由于脉冲场梯度SE或Ste和/或NMR微成像以进行可视化。（3）对T2和扩散系数的同时分析系统：用于扩散测量的脉冲序列进行了修改，以使物种的分数保持在样品恒定中。（4）在存在化学质子交换和差分交换的情况下，T2的温度依赖性的理论处理：对由于水质子而导致的磁化时间的微分方程进行数值分析，以在实验条件下随温度变化而获得T2的可能变化。2。浓缩丙兰溶液中的水迁移能力：通过使用已建立的Systenn 1（1）和（3），冷冻水（T210μs），未散发水（T2100μs）和-11°处的惰性聚合物质子（T2100μms）（T2 1ms）相互区分，在温度下，水和自由水的差异更高，而不是在温度下差异化。证明了在水溶液中Pullulan链的灵活性3。通过Cu（II）EPR方法分析了通过Gellan Gum进行二价阳离子的吸收机制，并在分子水平上清楚地表明了该机理。4。根据 ^1H T_2 NMR，CD和粘弹性测量，研究了明胶凝胶化过程的动力学研究。在线圈形成之前，氢键宿主已证明。