Cytokine found in insects for the forsttime ; studies on its activation mechanism and multi function

首次在昆虫中发现细胞因子；

• 批准号: 11640674
• 负责人: HAYAKAWA Yoichi
• 金额: $ 2.3万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11640674/
• 关键词: Insects; cytokine; processing enzyme; growth-blocking peptide (GBP); stress; immunity; blood coagulation factor Xa; セリンプロテアーゼ; アミノ酸配列; 血球凝真反応; ファクター10; 血球凝集反応

Growth-blocking peptide (GBP) is a cytokine found in insects for the first time. GBP is synthesized as a precursor protein mainly in fat body and the proGBP processing enzyme activity is present in fat body of the armyworm larvae. We purified the proGBP processing enzyme, and found that it has similar substrate specificity with Factor Xa in mammals.

生长阻断肽（Growth-blocking peptide，GBP）是首次在昆虫中发现的一种细胞因子。GBP作为前体蛋白主要在脂肪体中合成，并且proGBP加工酶活性存在于粘虫幼虫的脂肪体中。我们纯化了proGBP加工酶，发现它与哺乳动物中的Xa因子具有相似的底物特异性。

项目成果

V.Kostal: "Induction and development of winter larval diapause in a drosophilia fly, Chymomyza costata"J. Insect Physiol. 461. 417-428 (2000)

V.Kostal：“果蝇，Chymomyza costata 冬季幼虫滞育的诱导和发育”J。

V.Kostal: "Circadian factor influences induction of seasonally expressed hibernal diapause in a chosophilia fly"J.Insect Physiol. (in press). (2000)

V.Kostal：“昼夜节律因素影响嗜食蝇季节性表达的冬眠滞育的诱导”J.Insect Physiol。

K.D. Clark, B.F. Volkman. H. Thoetkiattikul, D. King, Y. Hayakawa, M.R.: "Strand Alanine-scanning mutagenesis of plasmatocyte spreading peptide identifies critical residues for biological activity"J. Biol. Chem.. 276. 18491-18496 (2001)

K.D.

M.Kamimura, Nakahara Y., Kanamori Y., Tsuzuki S., Hayakawa Y., Kiuti M.: "Molecular cloning of silkworm paralytic peptide and its developmental regulation"Biochem. Biophys. Res. Commun.. 286. 67-73 (2001)

M.Kamimura、Nakahara Y.、Kanamori Y.、Tsuzuki S.、Hayakawa Y.、Kiuti M.：“蚕麻痹肽的分子克隆及其发育调控”Biochem。

Strand, M.R.: "Plasmatocyte spreading peptide (PSP1) and growth blocking paptide (GBP) are multifunctional homologs."J. Insect Physiol.. 46. 817-824 (2000)

Strand, M.R.：“浆细胞扩散肽 (PSP1) 和生长阻断肽 (GBP) 是多功能同系物。”J.